PDF(Pubmed)
Abstract：
OBJECTIVE: In this study, we investigated the impact of perioperative administration of Bifidobacterium triplex viable capsules on the serum levels of circulating miR-21-5p, miR-135-5p, and miR-155-5p in patients with colorectal cancer (CRC). The purpose of this study is to provide a foundation for future research on the use of Bifidobacterium triplex viable capsules to enhance postoperative recovery in patients with CRC.
METHODS: A total of 60 patients with primary CRC admitted to the Department of General Surgery at Shanxi Bethune Hospital between June 2020 and December 2020 were selected and randomly divided into two groups: 20 cases in the control group and 40 cases in the experimental group. The experimental group was administered oral Bifidobacterium triplex viable capsules during the perioperative period, while the control group was administered oral placebo. Before and after the perioperative period, the expression levels of miR-21-5p, miR-135-5p, and miR-155-5p were compared in the serum of both groups of patients. Furthermore, we established the prognostic value of these three miRNAs in CRC patients.
RESULTS: After surgery, the expression levels of miR-21-5p, miR-135-5p, and miR-155-5p decreased in both groups of patients (P < 0.05). Significantly greater differences were observed between miR-21-5p and miR-135-5p (P < 0.001). Expression levels of serum miR-21-5p (P = 0.020) and miR-135-5p (P = 0.023) decreased significantly more in the experimental group than in the control group. The levels of the above three miRNAs after surgery did not correlate with 3-year OS (HR = 4.21; 95% CI 0.37-47.48; log-rank P = 0.20) or 3-year DFS (HR = 1.57; 95% CI 0.32-7.66; log-rank P = 0.55) in two groups.
CONCLUSIONS: Radical surgery reduces the levels of serum miR-21-5p, miR-135-5p, and miR-155-5p expression in patients with CRC. The use of Bifidobacterium triplex viable capsules assists in achieving quicker perioperative recovery from radical surgery in CRC patients, and this underlying mechanism may be associated with the regulation of serum miR-21-5p, miR-135-5p, and miR-155-5p expression levels.
METHODS: A total of 60 patients with primary CRC admitted to the Department of General Surgery at Shanxi Bethune Hospital between June 2020 and December 2020 were selected and randomly divided into two groups: 20 cases in the control group and 40 cases in the experimental group. The experimental group was administered oral Bifidobacterium triplex viable capsules during the perioperative period, while the control group was administered oral placebo. Before and after the perioperative period, the expression levels of miR-21-5p, miR-135-5p, and miR-155-5p were compared in the serum of both groups of patients. Furthermore, we established the prognostic value of these three miRNAs in CRC patients.
RESULTS: After surgery, the expression levels of miR-21-5p, miR-135-5p, and miR-155-5p decreased in both groups of patients (P < 0.05). Significantly greater differences were observed between miR-21-5p and miR-135-5p (P < 0.001). Expression levels of serum miR-21-5p (P = 0.020) and miR-135-5p (P = 0.023) decreased significantly more in the experimental group than in the control group. The levels of the above three miRNAs after surgery did not correlate with 3-year OS (HR = 4.21; 95% CI 0.37-47.48; log-rank P = 0.20) or 3-year DFS (HR = 1.57; 95% CI 0.32-7.66; log-rank P = 0.55) in two groups.
CONCLUSIONS: Radical surgery reduces the levels of serum miR-21-5p, miR-135-5p, and miR-155-5p expression in patients with CRC. The use of Bifidobacterium triplex viable capsules assists in achieving quicker perioperative recovery from radical surgery in CRC patients, and this underlying mechanism may be associated with the regulation of serum miR-21-5p, miR-135-5p, and miR-155-5p expression levels.
摘要：
目的：在本研究中，我们研究了围手术期服用双歧杆菌三联活菌胶囊对血清循环miR-21-5p水平的影响，miR-135-5p,和miR-155-5p在结直肠癌（CRC）患者中的表达。本研究的目的是为未来研究使用双歧杆菌三联活菌胶囊促进CRC患者术后恢复提供基础。
方法：选取2020年6月至2020年12月山西省白求恩医院普外科收治的60例原发性CRC患者，随机分为对照组20例和实验组40例。实验组在围手术期口服双歧杆菌三联活菌胶囊,对照组给予口服安慰剂。围手术期前后，miR-21-5p的表达水平，miR-135-5p,比较两组患者血清中的miR-155-5p。此外，我们确定了这三种miRNA在CRC患者中的预后价值.
结果：手术后，miR-21-5p的表达水平，miR-135-5p,两组患者的miR-155-5p均降低(P<0.05)。在miR-21-5p和miR-135-5p之间观察到显著更大的差异(P<0.001)。实验组血清miR-21-5p(P=0.020)和miR-135-5p(P=0.023)的表达水平较对照组明显下降。两组患者术后3年OS(HR=4.21;95%CI0.37~47.48;log-rankP=0.20)或3年DFS(HR=1.57;95%CI0.32~7.66;log-rankP=0.55)均无相关性。
结论：根治性手术可降低血清miR-21-5p水平，miR-135-5p,和miR-155-5p在CRC患者中的表达。双歧杆菌三联活菌胶囊的使用有助于CRC患者从根治性手术中获得更快的围手术期恢复。这种潜在的机制可能与血清miR-21-5p的调节有关,miR-135-5p,和miR-155-5p表达水平。
方法：选取2020年6月至2020年12月山西省白求恩医院普外科收治的60例原发性CRC患者，随机分为对照组20例和实验组40例。实验组在围手术期口服双歧杆菌三联活菌胶囊,对照组给予口服安慰剂。围手术期前后，miR-21-5p的表达水平，miR-135-5p,比较两组患者血清中的miR-155-5p。此外，我们确定了这三种miRNA在CRC患者中的预后价值.
结果：手术后，miR-21-5p的表达水平，miR-135-5p,两组患者的miR-155-5p均降低(P<0.05)。在miR-21-5p和miR-135-5p之间观察到显著更大的差异(P<0.001)。实验组血清miR-21-5p(P=0.020)和miR-135-5p(P=0.023)的表达水平较对照组明显下降。两组患者术后3年OS(HR=4.21;95%CI0.37~47.48;log-rankP=0.20)或3年DFS(HR=1.57;95%CI0.32~7.66;log-rankP=0.55)均无相关性。
结论：根治性手术可降低血清miR-21-5p水平，miR-135-5p,和miR-155-5p在CRC患者中的表达。双歧杆菌三联活菌胶囊的使用有助于CRC患者从根治性手术中获得更快的围手术期恢复。这种潜在的机制可能与血清miR-21-5p的调节有关,miR-135-5p,和miR-155-5p表达水平。
