下丘脑是动物中重要的神经内分泌区域,可以调节性发育。长非编码RNA(lncRNAs)被假定为调节与动物繁殖相关的生理过程。然而,lncRNAs参与山羊性成熟的调控机制知之甚少,特别是从出生到性成熟。在这项研究中,对四个发育阶段的下丘脑进行RNAseq分析(1天(D1,n=5),2个月(M2,n=5),4个月(M4,n=5),济宁灰山羊6个月(M6,n=5))。结果显示在下丘脑中鉴定出总共237个差异表达的lncRNAs(DELs)。其中,221个DEL对693个靶基因表现出顺式调节作用,而24个DEL对63个靶基因表现出反式调节作用。这些DELs的靶基因主要参与与能量代谢相关的生物过程,信号转导和激素分泌,如鞘脂信号通路,脂肪细胞因子信号通路,神经营养信号通路,谷氨酸能突触,P53信号通路和GnRH信号通路。此外,XR_001918477.1、TCONS_00077463、XR_001918760.1和TCONS_00029048及其潜在的靶基因可能在山羊性成熟过程中起着至关重要的作用。这项研究促进了我们对山羊性成熟过程中下丘脑组织中lncRNA的理解,并将为改善山羊生殖特征提供理论基础。
The
hypothalamus is an essential neuroendocrine area in animals that regulates sexual development. Long non-coding RNAs (lncRNAs) are hypothesized to regulate physiological processes related to animal reproduction. However, the regulatory mechanism by which lncRNAs participate in sexual maturity in goats is poorly known, particularly from birth to sexual maturation. In this study, RNAseq analysis was conducted on the
hypothalamus of four developmental stages (1day (D1, n = 5), 2 months (M2, n = 5), 4 months (M4, n = 5), and 6 months (M6, n = 5)) of Jining grey goats. The results showed that a total of 237 differentially expressed lncRNAs (DELs) were identified in the
hypothalamus. Among these, 221 DELs exhibited cis-regulatory effects on 693 target genes, while 24 DELs demonstrated trans-regulatory effects on 63 target genes. The target genes of these DELs are mainly involved in biological processes related to energy metabolism, signal transduction and hormone secretion, such as sphingolipid signaling pathway, adipocytokine signaling pathway, neurotrophic signaling pathway, glutamatergic synapse, P53 signaling pathway and GnRH signaling pathway. In addition, XR_001918477.1, TCONS_00077463, XR_001918760.1, and TCONS_00029048 and their potential target genes may play a crucial role in the process of goat sexual maturation. This study advances our understanding of lncRNA in hypothalamic tissue during sexual maturation in goats and will give a theoretical foundation for improving goat reproductive features.