■本研究旨在对2型糖尿病(T2D)患者的血清氨基酸谱及其相关并发症进行全面调查。
■T2D患者纳入本研究。采用液相色谱-质谱联用(LC-MS)法检测空腹循环中常见的16种氨基酸。随后,相关性,回归分析,和受试者工作特征(ROC)曲线,以评估氨基酸与临床指标之间的关联。
■在糖尿病患者中鉴定出13种不同的氨基酸,与正常对照组相比。谷氨酰胺/谷氨酸(Gln/Glu)比值与BMI呈负相关,HbA1c,血清尿酸,和甘油三酯-葡萄糖(TyG)指数,而与HDL-C呈正相关。Logistic回归分析表明,Gln/Glu是T2D(OR=0.65,95%CI0.50-0.86)和肥胖(OR=0.79,95%CI0.66-0.96)的一致保护因素。ROC曲线表明Gln/Glu,脯氨酸,缬氨酸,亮氨酸为糖尿病风险提供了有效的预测,Gln/Glu表现出最高的AUC[0.767(0.678-0.856)]。在T2D患者中,Gln是唯一与HbA1c呈负相关的氨基酸(r=-0.228,p=0.017)。此外,HOMA-β与Glu呈负相关(r=-0.301,p=0.003),与Gln/Glu呈正相关(r=0.245,p=0.017)。值得注意的是,logistic回归分析显示Gln/Glu与糖尿病肾病风险呈负相关(OR=0.74,95%CI0.55-0.98),与糖尿病视网膜病变风险呈正相关(OR=1.53,95%CI1.08-2.15)。
■Gln/Glu比值与糖尿病显著相关,常见的代谢参数,和糖尿病并发症。这些发现揭示了Gln代谢在T2D及其相关并发症中的关键作用。
UNASSIGNED: This study aims to conduct a comprehensive investigation of the serum amino acid profiles of individuals with type 2 diabetes (T2D) and its related complications.
UNASSIGNED: Patients with T2D were enrolled in this study. Sixteen kinds of common amino acids in the fasting circulating were assessed through liquid chromatography-mass spectrometry (LC-MS). Subsequently, correlation, regression analyses, and receiver operating characteristic (ROC) curves were conducted to assess the associations between amino acids and clinical indicators.
UNASSIGNED: Thirteen different kinds of amino acids were identified in diabetic patients, as compared with normal controls. The
Glutamine/Glutamate (Gln/Glu) ratio was negatively correlated with BMI, HbA1c, serum uric acid, and the triglyceride-glucose (TyG) index, while it was positively correlated with HDL-C. Logistic regression analyses indicated that Gln/Glu was a consistent protective factor for both T2D (OR = 0.65, 95% CI 0.50-0.86) and obesity (OR = 0.79, 95% CI 0.66-0.96). The ROC curves demonstrated that Gln/Glu, proline, valine, and leucine provided effective predictions for diabetes risk, with Gln/Glu exhibiting the highest AUC [0.767 (0.678-0.856)]. In patients with T2D, Gln was the only amino acid that displayed a negative correlation with HbA1c (r = -0.228, p = 0.017). Furthermore, HOMA-β exhibited a negative correlation with Glu (r = -0.301, p = 0.003) but a positive correlation with Gln/Glu (r = 0.245, p = 0.017). Notably, logistic regression analyses revealed an inverse correlation of Gln/Glu with the risk of diabetic kidney disease (OR = 0.74, 95% CI 0.55-0.98) and a positive association with the risk of diabetic retinopathy (OR = 1.53, 95% CI 1.08-2.15).
UNASSIGNED: The Gln/Glu ratio exhibited a significant association with diabetes, common metabolic parameters, and diabetic complications. These findings shed light on the pivotal role of Gln metabolism in T2D and its associated complications.