保护血管壁完整性,防止与衰老相关的退行性过程,富含脂肪的饮食和代谢性疾病是及时治疗的挑战。内皮功能和完整性的丧失导致心血管疾病和多器官炎症。H2S供体芥酸素的保护作用,一种异硫氰酸酯,由芥子磨坊纯化。种子,在人内皮细胞和血管平滑肌细胞上进行了评估。特别是,对细胞活力评估芥酸素的作用,ROS,caspase3/7,炎症标志物水平和与高葡萄糖浓度(25mM,HG)。Erucin显着阻止HG诱导的细胞活力降低以及ROS的增加,caspase3/7激活,TNF-α和IL-6水平。同样,芥酸素抑制与HG暴露相关的COX-2和NF-κB上调。Erucin还引起内皮细胞中p22phox亚基表达的显著抑制。此外,通过定量特异性标记物VE-Cadherin和ZO-1也证实了芥酸显著阻止HG诱导的内皮通透性增加。总之,我们的结果评估了芥酸素在经历HG诱导的炎症的血管细胞中的抗炎和抗氧化作用,这种保护作用与内皮屏障特性的保持平行.
Preservation of vascular wall integrity against degenerative processes associated with ageing, fat-rich diet and metabolic diseases is a timely therapeutical challenge. The loss of endothelial function and integrity leads to cardiovascular diseases and multiorgan inflammation. The protective effects of the H2S-donor
erucin, an isothiocyanate purified by Eruca sativa Mill. seeds, were evaluated on human endothelial and vascular smooth muscle cells. In particular,
erucin actions were evaluated on cell viability, ROS, caspase 3/7, inflammatory markers levels and the endothelial hyperpermeability in an inflammatory model associated with high glucose concentrations (25 mM, HG).
Erucin significantly prevented the HG-induced decrease in cell viability as well as the increase in ROS, caspase 3/7 activation, and TNF-α and IL-6 levels. Similarly,
erucin suppressed COX-2 and NF-κB upregulation associated with HG exposure.
Erucin also caused a significant inhibition of p22phox subunit expression in endothelial cells. In addition, erucin significantly prevented the HG-induced increase in endothelial permeability as also confirmed by the quantification of the specific markers VE-Cadherin and ZO-1. In conclusion, our results assess anti-inflammatory and antioxidant effects by erucin in vascular cells undergoing HG-induced inflammation and this protection parallels the preservation of endothelial barrier properties.