PDF(Pubmed)
Abstract：
The study on Erucin (ER) has gained interest of nutraceutical and pharmaceutical industries because of its anti-cancer properties. Erucin is an isothiocyanate obtained from the seeds of Eruca sativa which possess certain drawbacks such as poor aqueous solubility and bioavailability. Therefore, the present study aimed at developing ER-cubosomes (CUB) by solvent evaporation technique followed by applying Central Composite Design to optimize ER loaded cubosomes. For this purpose, independent variables selected were Monoolein (MO) as lipid and Pluronic-84 (P-84) as a stabilizer whereas dependent variables were particle size, percentage of ER loading and percentage of its entrapment efficiency. The cubosomal nanocarriers exhibited particle size in the range of 26 nm, entrapment efficiency of 99.12 ± 0.04% and drug loading of 3.96 ± 0.0001%. Furthermore, to investigate the antioxidant potential, we checked the effect of ER and ER-CUB by DNA nicking assay, DDPH assay and Phosphomolybdate assay, and results showed significant improvement in antioxidant potential for ER-CUB than ER. Similarly, ER-CUB showed enhanced anticancer activity with a marked reduction in IC50 value than ER in MTT assay. These results suggested that ER-CUB produced notable escalation in antioxidant potential and enhanced anticancer activity than ER.
摘要：
由于Erucin（ER）的抗癌特性，对Erucin（ER）的研究引起了营养食品和制药行业的兴趣。芥酸素是由芥酸种子获得的异硫氰酸酯,其具有某些缺点,例如差的水溶性和生物利用度。因此,本研究旨在通过溶剂蒸发技术开发ER-立方体（CUB），然后应用中央复合材料设计来优化ER负载的立方体。为此,选择的独立变量是作为脂质的单油酸甘油酯（MO）和作为稳定剂的Pluronic-84（P-84），而因变量是粒径，ER负载百分比及其截留效率百分比。立方体纳米载体的粒径范围为26nm，包封率为99.12±0.04%,载药量为3.96±0.0001%。此外，为了研究抗氧化潜力，我们通过DNA切口分析检查了ER和ER-CUB的作用，DDPH测定和磷钼酸盐测定，结果表明，ER-CUB的抗氧化潜力比ER显着提高。同样，在MTT分析中，ER-CUB显示出增强的抗癌活性，IC50值比ER明显降低。这些结果表明，ER-CUB比ER产生了显着的抗氧化潜力和增强的抗癌活性。
