背景:特应性疾病,肥胖和神经精神疾病是与生活方式和环境相关的慢性炎症性疾病,发病率在过去几年有所增加。
目的:概述哥本哈根儿童哮喘前瞻性研究(COPSAC2000)出生队列的18年随访设计,特应性疾病的危险因素,肥胖和神经精神疾病是通过对环境的广泛表征来识别的,以及用于组学分析的深度临床表型和生物采样。
方法:COPSAC2000是一项丹麦前瞻性临床出生队列研究,研究对象为411名患有哮喘的母亲所生儿童,这些儿童在1月龄时被纳入,并在COPSAC临床研究单位密切跟踪,直至儿童期发展特应性疾病。在18年的随访中,生物材料(头发,血,尿液,粪便,喉咙,还有皮肤拭子,鼻衬液和刮擦,和下咽抽吸物)和关于环境暴露和风险行为的广泛信息,以及深层代谢特征和多器官调查,包括人体测量学,心,肺,肾脏,肠子,骨头,肌肉和皮肤。神经精神诊断是从医疗记录和登记册中获取的,并附有有关行为特征和精神病理学的电子问卷。
结果:411名队列参与者中,共有370人(90%)完成了18年的访视。其中,25.1%有哮喘,23.4%的人体重指数>25kg/m2,16.8%的人在儿童时期有精神病诊断。在儿童时期有神经精神病学诊断的62名先证中,共有68.7%的人每月饮酒,喝酒的时候,22.2%的人饮用>10单位。在参与者中,31.4%的人目前吸烟,其中,每天吸烟24.1%。共有23.8%的人曾尝试吸毒,19.7%的人报告有自我毁灭行为。每天的平均筛选时间为6.0小时。
结论:这个关于健康和习惯的巨大数据集,暴露,新陈代谢,18岁时COPSAC2000的多器官评估和生物样本为探索特应性疾病和其他生活方式相关的风险因素和潜在机制提供了独特的机会。非传染性疾病,如肥胖症和神经精神疾病,在社区和我们的队列中非常普遍。
BACKGROUND: Atopic diseases, obesity and neuropsychiatric disorders are lifestyle-related and environmental-related chronic inflammatory disorders, and the incidences have increased in the last years.
OBJECTIVE: To outline the design of the 18-year follow-up of the Copenhagen Prospective Study on Asthma in Childhood (COPSAC2000) birth cohort, where risk factors of atopic diseases, obesity and neuropsychiatric disorders are identified through extensive characterisation of the environment, along with deep clinical phenotyping and biosampling for omics profiling.
METHODS: COPSAC2000 is a Danish prospective clinical birth cohort study of 411 children born to mothers with asthma who were enrolled at 1 month of age and closely followed at the COPSAC clinical research unit through childhood for the development of atopic diseases. At the 18-year follow-up visit, biomaterial (hair, blood, urine, faeces, throat, and skin swabs, nasal lining fluid and scraping, and hypopharyngeal aspirates) and extensive information on environmental exposures and risk behaviours were collected along with deep metabolic characterisation and multiorgan investigations including anthropometrics, heart, lungs, kidneys, intestines, bones, muscles and skin. Neuropsychiatric diagnoses were captured from medical records and registers accompanied by electronic questionnaires on behavioural traits and psychopathology.
RESULTS: A total of 370 (90%) of the 411 cohort participants completed the 18-year visit. Of these, 25.1% had asthma, 23.4% had a body mass index >25 kg/m2 and 16.8% had a psychiatric diagnosis in childhood. Of the 62 probands with a neuropsychiatric diagnosis in childhood, a total of 68.7% drank alcohol monthly, and when drinking, 22.2% drank >10 units. Of the participants, 31.4% were currently smoking, and of these, 24.1% smoked daily. A total of 23.8% had tried taking drugs, and 19.7% reported having done self-destructive behaviour. The mean screen time per day was 6.0 hours.
CONCLUSIONS: This huge dataset on health and habits, exposures, metabolism, multiorgan assessments and biosamples from COPSAC2000 by age 18 provides a unique opportunity to explore risk factors and underlying mechanisms of atopic disease and other lifestyle-related, non-communicable diseases such as obesity and neuropsychiatric disorders, which are highly prevalent in the community and our cohort.