■为了研究茶黄素-3,3'-二烷基酯(TF3)/乙醇溶液交联脱矿质牙本质胶原蛋白的能力,抵抗胶原酶的消化,并探索潜在的机制。
■使用无龋齿的人第三磨牙制备完全去矿化的牙本质块。然后,这些块被随机分配到14个单独的组(n=6),即,control,乙醇,5%戊二醛(GA),12.5、25、50和100mg/mlTF3/乙醇溶液组。根据交联时间将每组进一步分为两个亚组:30和60s。通过分子对接预测TF3与牙本质I型胶原相互作用的功效和机制。交联,抗酶降解,通过减肥研究生物力学特性,羟脯氨酸释放,扫描/透射电子显微镜(SEM/TEM),原位酶谱,表面硬度,热重分析,和溶胀率。傅里叶变换红外光谱(FTIR),X射线光电子能谱(XPS),和拉曼光谱被用来探索其机制。使用单向和双向方差分析和Tukey检验进行统计学分析。
■TF3/乙醇溶液能有效交联脱矿质牙本质胶原,提高其抗胶原酶消化能力和生物力学特性(p<0.05),显示浓度和时间依赖性。25和50mg/mlTF3/乙醇溶液的效果与5%GA相似,而100mg/mLTF3/乙醇溶液表现出更好的性能(p<0.05)。TF3和牙本质Ⅰ型胶原主要通过氢键交联,并且可能存在共价和疏水相互作用。
■TF3具有有效交联脱矿质牙本质胶原蛋白的能力,在临床可接受的时间范围内(30s/60s)增强其对胶原酶酶促水解和生物力学特性的抵抗力。此外,它在延长牙本质粘附寿命方面表现出希望。
UNASSIGNED: To study the ability of theaflavin-3,3\'-digallate (TF3)/ethanol solution to crosslink demineralized dentin collagen, resist collagenase digestion, and explore the potential mechanism.
UNASSIGNED: Fully demineralized dentin blocks were prepared using human third molars that were caries-free. Then, these blocks were randomly allocated into 14 separate groups (n = 6), namely, control, ethanol, 5% glutaraldehyde (GA), 12.5, 25, 50, and 100 mg/ml TF3/ethanol solution groups. Each group was further divided into two subgroups based on crosslinking time: 30 and 60 s. The efficacy and mechanism of TF3\'s interaction with dentin type I collagen were predicted through molecular docking. The cross-linking, anti-enzymatic degradation, and biomechanical properties were studied by weight loss, hydroxyproline release, scanning/transmission electron microscopy (SEM/TEM), in situ zymography, surface hardness, thermogravimetric analysis, and swelling ratio. Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy were utilized to explore its mechanisms. Statistical analysis was performed using one and two-way analysis of variance and Tukey\'s test.
UNASSIGNED: TF3/ethanol solution could effectively crosslink demineralized dentin collagen and improve its resistance to collagenase digestion and biomechanical properties (p < 0.05), showing concentration and time dependence. The effect of 25 and 50 mg/ml TF3/ethanol solution was similar to that of 5% GA, whereas the 100 mg/mL TF3/ethanol solution exhibited better performance (p < 0.05). TF3 and dentin type I collagen are mainly cross-linked by hydrogen bonds, and there may be covalent and hydrophobic interactions.
UNASSIGNED: TF3 has the capability to efficiently cross-link demineralized dentin collagen, enhancing its resistance to collagenase enzymatic hydrolysis and biomechanical properties within clinically acceptable timeframes (30 s/60 s). Additionally, it exhibits promise in enhancing the longevity of dentin adhesion.