该研究描述了两种抗生素抗性代表性大肠杆菌菌株的全基因组测序,2020年从家禽粪便中分离出来。样品从波兰的商业鸡肉生产设施获得。抗生素耐药性特征在于对β-内酰胺抗生素的共同耐药性,氨基糖苷类,和氟喹诺酮类药物.三个鉴定的抗性质粒(R-质粒),pECmdr13.2,pECmdr13.3和pECmdr14.1具有各种赋予四环素抗性的基因(tetR[A]),氨基糖苷(aph,aac,和aad家庭),β-内酰胺(blaCMY-2,blaTEM-176),磺酰胺(sul1,sul2),氟喹诺酮(qnrS1),和苯酚(floR)。这些质粒,波兰以前没有报道过,被发现带有IS26插入元件,intI1-整合酶基因,和夫妻间转移基因,促进水平基因转移。质粒pECmdr13.2和pECmdr14.1还具有与转座子Tn6196相关的汞抗性基因操纵子;由于共选择机制如共抗性,即使没有抗生素选择压力,这也会促进质粒的持久性。鸡粪来源的质粒属于IncX1(窄宿主范围)和IncC(宽宿主范围)不相容组。已经在各种环境中鉴定出类似的质粒,临床分离株,农场动物,包括牛,猪,和家禽。这项研究对“一个健康”方法具有重要意义,因为它突出了来自牲畜和食物来源的抗生素抗性细菌的潜力,特别是大肠杆菌,通过食物链转移给人类,反之亦然。
The study describes the whole-genome sequencing of two antibiotic-resistant representative Escherichia coli strains, isolated from poultry manure in 2020. The samples were obtained from a commercial chicken meat production facility in Poland. The antibiotic resistance profile was characterized by co-resistance to β-lactam antibiotics, aminoglycosides, and fluoroquinolones. The three identified resistance plasmids (R-plasmids), pECmdr13.2, pECmdr13.3, and pECmdr14.1, harbored various genes conferring resistance to tetracyclines (tetR[A]) for, aminoglycoside (aph, aac, and aad families), β-lactam (blaCMY-2, blaTEM-176), sulfonamide (sul1, sul2), fluoroquinolone (qnrS1), and phenicol (floR). These plasmids, which have not been previously reported in Poland, were found to carry IS26 insertion elements, the intI1-integrase gene, and conjugal transfer genes, facilitating horizontal gene transfer. Plasmids pECmdr13.2 and pECmdr14.1 also possessed a mercury resistance gene operon related to transposon Tn6196; this promotes plasmid persistence even without antibiotic selection pressure due to co-selection mechanisms such as co-resistance. The chicken manure-derived plasmids belonged to the IncX1 (narrow host range) and IncC (broad host range) incompatibility groups. Similar plasmids have been identified in various environments, clinical isolates, and farm animals, including cattle, swine, and poultry. This study holds significant importance for the One Health approach, as it highlights the potential for antibiotic-resistant bacteria from livestock and food sources, particularly E. coli, to transfer through the food chain to humans and vice versa.