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Abstract：
Leach bed reactors (LBRs) are dry anaerobic systems that can handle feedstocks with high solid content, like chicken manure, with minimal water addition. In this study, the chicken manure was mixed with zeolite, a novel addition, and packed in the LBR to improve biogas production. The resulting leachate was then processed in a continuous stirred tank reactor (CSTR), where most of the methane was produced. The supernatant of the CSTR was returned to the LBR. The batch mode operation of the LBR led to a varying methane production rate (MPR) with a peak in the beginning of each batch cycle when the leachate was rich in organic matter. Comparing the MPR in both systems, the peaks in the zeolite system were higher and more acute than in the control system, which was under stress, as indicated by the acetate accumulation at 2328 mg L-1. Moreover, the presence of zeolite in the LBR played a crucial role, increasing the overall methane yield from 0.142 (control experiment) to 0.171 NL CH4 per g of volatile solids of chicken manure entering the system at a solid retention time of 14 d. Zeolite also improved the stability of the system. The ammonia concentration increased gradually due to the little water entering the system and reached 3220 mg L-1 (control system) and 2730 mg L-1 (zeolite system) at the end of the experiment. It seems that zeolite favored the accumulation of the ammonia at a lower rate (14.0 mg L-1 d-1) compared to the control experiment (17.3 mg L-1 d-1). The microbial analysis of the CSTR fed on the leachate from the LBR amended with zeolite showed a higher relative abundance of Methanosaeta (83.6%) compared to the control experiment (69.1%). Both CSTRs established significantly different bacterial profiles from the inoculum after 120 days of operation (p < 0.05). Regarding the archaeal communities, there were no significant statistical differences between the CSTRs and the inoculum (p > 0.05).
摘要：
浸出床反应器（LBR）是干式厌氧系统，可以处理高固体含量的原料，比如鸡粪,添加最少的水。在这项研究中,鸡粪与沸石混合，一个新颖的补充，并包装在LBR中，以提高沼气产量。然后在连续搅拌釜反应器（CSTR）中处理所得的渗滤液，大部分甲烷是在那里产生的。将CSTR的上清液返回到LBR。LBR的分批模式操作导致甲烷生产率（MPR）变化，当渗滤液富含有机物时，在每个分批循环开始时达到峰值。比较两个系统中的MPR，沸石系统中的峰比控制系统中的峰更高且更尖锐，在压力下，如2328mgL-1的乙酸积累所示。此外，沸石在LBR中的存在起着至关重要的作用,在固体停留时间为14d时，将每克鸡粪挥发性固体的总甲烷产量从0.142（对照实验）提高到0.171NLCH4。沸石还提高了系统的稳定性。由于进入系统的水很少，氨浓度逐渐增加，在实验结束时达到3220mgL-1（控制系统）和2730mgL-1（沸石系统）。与对照实验（17.3mgL-1d-1）相比，沸石似乎以较低的速率（14.0mgL-1d-1）促进氨的积累。对来自用沸石修正的LBR的渗滤液的CSTR的微生物分析显示，与对照实验（69.1％）相比，甲烷的相对丰度更高（83.6％）。两种CSTR在手术120天后建立了与接种物显著不同的细菌谱(p<0.05)。关于考古社区，CSTRs与接种物之间无显著统计学差异(p>0.05)。
