■凯尔血型系统在输血医学中具有重要的临床意义,特别是在具有Kell抗原特异性抗体的患者中。迄今为止,泰国人群中Kell金属内肽酶(KEL)基因的遗传变异仍然未知。
■本研究旨在使用内部聚合酶链反应序列特异性引物(PCR-SSP)方法确定泰国献血者中KEL*03和KEL*04等位基因的频率。
■在Pathumthani的一家血库中,从805名无关的中央泰国献血者获得的血液样本,泰国,从2023年3月到2023年6月,使用柱凝集试验对Kpa和Kpb抗原进行分型,并且使用DNA测序确认了400个样本的结果。建立了检测KEL*03和KEL*04等位基因的PCR-SSP方法,和基因分型结果使用已知的DNA对照进行验证。从中央的泰国捐献者获得的DNA样本(n=2529),北方(n=300),和泰国南部(n=427)也使用PCR-SSP进行基因分型以进行比较。
■所有805个(100%)供体具有Kp(a-b+)表型。PCR-SSP基因分型结果与柱凝集试验和DNA测序结果一致。所有3256名泰国献血者均具有纯合KEL*04/KEL*04基因型。泰国捐献者中KEL*03和KEL*04等位基因的频率与日本人显著不同,美洲原住民,南非,巴西,瑞士人,德国人口。
■这项研究在三个泰国人群中发现了100%的KEL*04等位基因频率。这些数据可以提供有关KEL*03和KEL*04等位基因频率的信息,以估计泰国人群中同种免疫的风险。
■这项研究表明,内部PCR-SSP可用于确定KEL*03和KEL*04等位基因,以预测Kpa和Kpb抗原。即使只有纯合的KEL*04/KEL*04基因型在泰国供体人群中发现,已建立的PCR-SSP方法可能有助于评估其他人群的同种免疫风险.
UNASSIGNED: The Kell blood group system is clinically important in transfusion medicine, particularly in patients with antibodies specific to Kell antigens. To date, genetic variations of the Kell metallo-endopeptidase (KEL) gene among Thai populations remain unknown.
UNASSIGNED: This study aimed to determine the frequencies of KEL*03 and KEL*04 alleles among Thai blood donors using an in-house polymerase chain reaction-sequence-specific primer (PCR-SSP) method.
UNASSIGNED: Blood samples obtained from 805 unrelated central Thai blood donors at a blood bank in Pathumthani, Thailand, from March 2023 to June 2023, were typed for Kpa and Kpb antigens using the column agglutination test, and the results for 400 samples were confirmed using DNA sequencing. A PCR-SSP method was developed to detect the KEL*03 and KEL*04 alleles, and genotyping results were validated using known DNA controls. DNA samples obtained from Thai donors in central (n = 2529), northern (n = 300), and southern (n = 427) Thailand were also genotyped using PCR-SSP for comparison.
UNASSIGNED: All 805 (100%) donors had the Kp(a-b+) phenotype. The PCR-SSP genotyping results agreed with the column agglutination test and DNA sequencing. All 3256 Thai blood donors had the homozygous KEL*04/KEL*04 genotype. Frequencies of the KEL*03 and KEL*04 alleles among Thai donors differed significantly from those of Japanese, Native American, South African, Brazilian, Swiss, and German populations.
UNASSIGNED: This study found a 100% KEL*04
allele frequency in three Thai populations. These data could provide information on KEL*03 and KEL*04
allele frequencies to estimate the risk of alloimmunisation in Thai populations.
UNASSIGNED: This study demonstrates that in-house PCR-SSP can be used to determine KEL*03 and KEL*04 alleles to predict Kpa and Kpb antigens. Even though only homozygous KEL*04/KEL*04 genotypes were found among Thai donor populations, the established PCR-SSP method may be useful for estimating the risk of alloimmunisation in other populations.