目的:哮喘是常见的,慢性,特应性呼吸道疾病在全世界儿童和成人中呈上升趋势。各种环境,遗传,和生物相互作用导致对这种疾病的易感性激增。白细胞介素(IL)基因,特别是IL-4和IL-13与哮喘发病机制有关。本研究旨在调查遗传畸变,特别是IL-4和IL-13的单核苷酸多态性(SNP),以及它们与儿童哮喘及其严重程度的关系。
方法:在阿萨姆邦的一家三级医院进行了一项以医院为基础的病例对照研究,印度。使用EpiInfo软件7.2版(疾病控制和预防中心,亚特兰大,GA,美国),假设置信区间为95%,这项研究的功率为80%,对照组与病例的比例为1,暴露量为22%的对照组比例,暴露病例的比例为46%。共有53例年龄在3至12岁之间的儿童哮喘临床诊断病例和39例未患有呼吸系统疾病且没有哮喘和/或过敏史的健康对照者在三级护理医院就诊。从未患有哮喘或过敏的儿童,并且在过去四周内没有任何上呼吸道或下呼吸道感染的儿童被视为对照。获得事先知情同意和伦理许可。非常严重的病例和对照被排除在研究之外。基因调查使用聚合酶链反应(PCR),其次是限制性片段长度多态性(RFLP),发现IL-4和IL-13基因中的SNP。对IL-13基因的+2044G>A与疾病严重程度相关的病例进行测序分析。使用χ2检验分析病例和对照之间特定SNP比例的差异(P值<0.05被认为是显著的)。
结果:IL-4的rs2070874和rs2243250多态性均无统计学意义。IL-13基因1111C>T的突变在病例中高于对照组。IL-13基因的+2044G>A多态性的基因型和等位基因分布均显示出与疾病严重程度的显着关联(p<0.05)。
结论:在研究区域的儿科患者中,IL-4和IL-13SNP的遗传畸变是普遍存在的。IL-13的SNP+2044G>A在研究区域的儿科人群中的疾病表现和严重程度中起作用。
OBJECTIVE: Asthma is a common, chronic, atopic respiratory disease that is on the rise among children and adults worldwide. Various environmental, genetic, and biological interactions contribute to the surge in susceptibility to this disease. Interleukin (IL) genes, particularly IL-4 and IL-13, have been linked to asthma pathogenesis. The present study aims to investigate the genetic aberrations, specifically single nucleotide polymorphisms (SNPs) of IL-4 and IL-13, and their association with childhood asthma and its severity.
METHODS: An unmatched hospital-based
case-control study was conducted in a tertiary care hospital in Assam, India. The sample size was calculated to be 120 (60 cases and 60 controls) using the Epi Info software version 7.2 (Centers for Disease Control and Prevention, Atlanta, GA, USA), assuming a confidence interval of 95%, a power of the study at 80%, a ratio of control to cases as 1, a proportion of controls with exposure at 22%, and a proportion of cases with exposure at 46%. A total of 53 clinically diagnosed cases of childhood asthma in the age range of three to 12 years and 39 healthy controls free from respiratory diseases and having no history of asthma and/or allergy of the same age group attending a tertiary care hospital were included in the study. Children who never had asthma or allergies and who did not suffer from any upper or lower respiratory infections for the previous four weeks were considered controls. Prior informed consent and ethical clearance were obtained. Very seriously ill cases and controls were excluded from the study. The genetic investigation used polymerase chain reaction (PCR), followed by restriction fragment length polymorphism (RFLP), to discover SNPs in the IL-4 and IL-13 genes. Sequencing analysis was done for the cases with +2044 G>A of the IL-13 gene in relation to the severity of the disease. The difference in the proportions of specific SNPs between cases and controls was analyzed using the χ2 test (a p-value of <0.05 was considered significant).
RESULTS: Both the rs2070874 and rs2243250 polymorphisms of IL-4 showed no statistically significant associations. The mutation of the IL-13 gene in 1111C>T was higher among cases than controls. Both genotypic and allelic distributions of the +2044G>A polymorphism of the IL-13 gene revealed a significant association (p<0.05) with the severity of the disease.
CONCLUSIONS: Genetic aberrations in SNPs of IL-4 and IL-13 are prevalent among the pediatric patients of the study region. The SNP +2044G>A of IL-13 is instrumental in disease manifestation and severity among the pediatric population of the study region.