目的:干燥引起的润唇膏表面能量的降低由于强疏水胶体-表面相互作用的出现而增强了病毒的粘附。
方法:开发了一种用于制备唇膏涂层的方案,以进行物理化学表征和附着力研究。测量了四种品牌的润唇膏(干燥和水合)和人腺病毒5(HAdV5)的表面电荷和疏水性,并用于计算润唇膏涂层与HAdV5之间相互作用的扩展的Derjaguin-Landau-Verwey-Overbeek(XDLVO)能量以及其他四种噬菌体:HAdV40,MS2和P22噬菌体,和SiO2。使用SiO2胶体进行耗散监测的石英晶体微天平(QCM-D)测试,HAdV5和水合润唇膏。
结果:润唇膏的干燥导致其表面能显著降低(δΔGsws≥83.0mJ/m2),使得表面高度疏水。对于干润唇膏,唇膏表面与所有五种胶体的相互作用是有吸引力的。对于在150mMNaCl(人类唾液的离子强度)中水合的润唇膏,XDLVO计算预测亲水胶体(MS2,P22,SiO2)可能会附着在浅的次要最小值中。由于人类腺病毒的相对疏水性,XDLVO剖面中的初级最大值较低或不存在,使得不可逆地沉积成初级能量最小值成为可能。使用SiO2胶体和HAdV5的初步QCM-D测试证实了在水合润唇膏上的沉积。
OBJECTIVE: Drying-induced decrease in lip balm surface energy enhances virus adhesion due to the emergence of strong hydrophobic colloid-surface interactions.
METHODS: A protocol was developed for preparing lip balm coatings to enable physicochemical characterization and adhesion studies. Surface charge and hydrophobicity of four brands of lip balm (dry and hydrated) and human adenovirus 5 (HAdV5) were measured and used to calculate the extended Derjaguin-Landau-Verwey-Overbeek (
XDLVO) energy of interactions between lip balm coatings and HAdV5 as well as four other colloids: HAdV40, MS2 and P22 bacteriophages, and SiO2. Quartz crystal microbalance with dissipation monitoring (QCM-D) tests employed SiO2 colloids, HAdV5 and hydrated lip balms.
RESULTS: Drying of lip balms results in a dramatic decrease of their surface energy (δΔGsws≥ 83.0 mJ/m2) making the surfaces highly hydrophobic. For dry lip balms, the interaction of the balm surface with all five colloids is attractive. For lip balms hydrated in 150 mM NaCl (ionic strength of human saliva),
XDLVO calculations predict that hydrophilic colloids (MS2, P22, SiO2) may attach into shallow secondary minima. Due to the relative hydrophobicity of human adenoviruses, primary maxima in
XDLVO profiles are low or non-existent making irreversible deposition into primary energy minima possible. Preliminary QCM-D tests with SiO2 colloids and HAdV5 confirm deposition on hydrated lip balms.