TOR(雷帕霉素靶)信号通路,以TOR激酶为核心,调节吸收,分布,通过整合代谢网络和其他信号通路来回收营养,从而参与植物生长-防御权衡。虽然萜类化合物在植物生长中起着重要作用,发展,应激反应,和信号转导。TOR信号通路对植物萜类生物合成的影响还有待详细研究。在这项研究中,用TOR抑制剂AZD8055处理丹参组培苗。结果表明,对照组的根在第8天已经开始生长,而用AZD8055处理的幼苗没有生根迹象。结合前8天TOR信号通路相关基因的表达变化,3号的样本,6th,选择第8天进行RNA-Seq分析。通过RNA-Seq分析,从这三个时期的样本中总共获得了50,689个基因,其中4088个基因表现出差异表达。差异表达基因(DEGs)的功能富集和时间序列分析表明,TOR被抑制后,随着处理时间的延长,TOR信号通路对植物生长相关过程的主要影响逐渐传递。DEGs通路富集分析表明,该基因在萜类化合物的生物合成中,如二萜和类胡萝卜素生物合成途径,可以被监管。与其他阶段相比,与萜类生物合成相关的DEGs主要在S2阶段受到调控。此外,参与萜类骨架生物合成的基因在S2阶段也相当丰富,根据基因集富集分析(GSEA)的结果对unigenes进行分析。抑制TOR信号通路可能影响萜类信号分子的生物合成,抑制赤霉素的生物合成,并促进脱落酸的生物合成。本研究从组学的角度探讨了干扰TOR途径对丹参萜类生物合成的影响,为萜类生物合成途径与药用植物生长-防御权衡之间的相互作用提供了新的见解。
The TOR (Target of Rapamycin) signaling pathway, which takes TOR kinase as the core, regulates the absorption, distribution, and recycling of nutrients by integrating metabolic network and other signaling pathways, thus participating in the plant growth-defense trade-off. While terpenoids play an important role in plant growth, development, stress response, and signal transduction. The effect of the TOR signaling pathway on terpenoid biosynthesis in plants has yet to be studied in detail. In this study, the tissue culture seedlings of Salvia miltiorrhiza were treated with the TOR inhibitor AZD8055. The results show that the roots of the control group had begun to grow on the 8th day, while the seedlings treated with AZD8055 had no rooting signs. Combined with the expression changes of genes related to the TOR signaling pathway in the first 8 days, samples on the 3rd, 6th, and 8th days were selected for RNA-Seq analysis. Through RNA-Seq analysis, a total of 50,689 unigenes were obtained from the samples of these three periods, of which 4088 unigenes showed differential expression. The function enrichment and time-series analysis of differentially expressed genes (DEGs) showed that the main influence of the TOR signal pathway on plant growth-related processes was gradually transmitted with treatment time after TOR was inhibited. Pathway enrichment analysis of DEGs showed that the genes in the biosynthesis of terpenoids, such as diterpenoid and carotenoid biosynthetic pathways, could be regulated. Compared with other stages, DEGs related to terpenoid biosynthesis were mainly regulated in the S2 stage. In addition, the genes involved in terpenoid skeleton biosynthesis was also considerably enriched in the S2 stage, according to the results of gene set enrichment analysis (GSEA) of unigenes. Inhibition of the TOR signaling pathway may affect the biosynthesis of terpenoid signaling molecules, inhibit gibberellin\'s biosynthesis, and promote abscisic acid\'s biosynthesis. This study has discussed the effect of interfering with the TOR pathway on terpenoid biosynthesis in S. miltiorrhiza from the perspective of omics and provides new insight into the interaction between the terpenoid biosynthesis pathway and the growth-defense trade-off of medicinal plants.