未经证实:格雷夫斯眼眶病(GO)是一种常见的自身免疫性疾病,但其具体发病机制尚未完全阐明。microRNAs(miRNAs)在自身免疫性疾病的发生发展中具有重要的调控功能。在本研究中,我们探讨了miR-182在GO诊断中的潜在作用。
UASSIGNED:通过qRT-PCR检测miR-182、促甲状腺激素受体(TSHR)和脂肪细胞因子的表达。用ELISA法测定甘油三酯(TG)含量。通过油红O染色鉴定脂滴含量。类E74因子3(ELF3)之间的关系miR-182和TSHR被ChIP证实,双荧光素酶报告基因测定和RIP。
UNASSIGNED:miR-182的表达降低,当TSHR增加时,和脂肪细胞因子(脂联素,瘦素,PPAR-γ,GO和差异培养基诱导(DM诱导)3T3-L1细胞患者的眶前脂肪组织中AP2)水平上调。MiR-182模拟物抑制脂肪细胞因子表达,TG含量和脂滴;然而,miR-182抑制剂具有相反的结果。TSHR是miR-182的靶基因,并且TSHR过表达(oe-TSHR)逆转了miR-182模拟物对DM处理的3T3-L1成脂分化的影响。ELF3转录促进miR-182表达。Oe-ELF3抑制DM诱导的3T3-L1细胞中脂肪细胞因子的表达并降低TG含量和脂滴。MiR-182抑制剂逆转oe-ELF3对3T3-L1成脂分化的影响。
未经证实:ELF3/miR-182/TSHR轴通过抑制脂肪分化减轻Graves眼眶病。
UNASSIGNED: Graves\' orbitopathy (GO) is a common autoimmune disease, but its specific pathogenesis has not been fully elucidated. MicroRNAs (miRNAs) possess an important regulatory function in the occurrence and development of autoimmune diseases. In the present study, we explored the potential role of miR-182 in the diagnosis of GO.
UNASSIGNED: The expression of miR-182, thyrotropin receptor (
TSHR) and adipocytokines was ascertained by qRT-PCR assay. The triglyceride (TG) content was ascertained by ELISA assay. The lipid droplet content was identified by Oil Red O staining. The relationship between E74-like factor 3 (ELF3), miR-182 and
TSHR was confirmed by ChIP, dual-luciferase reporter assay and RIP.
UNASSIGNED: The expression of miR-182 decreased, while
TSHR increased, and adipocytokine (adiponectin, leptin, PPAR-γ, and AP2) levels were upregulated in preorbital adipose tissue of patients with GO and differential medium induced (DM-induced) 3T3-L1 cells. MiR-182 mimics inhibited adipocytokine expression, TG content and lipid droplets; however, miR-182 inhibitor had the opposite results.
TSHR was a target gene of miR-182, and
TSHR overexpression (oe-
TSHR) reversed the effect of miR-182 mimics on adipogenic differentiation of 3T3-L1 by DM treatment. ELF3 transcription promoted miR-182 expression. Oe-ELF3 inhibited adipocytokine expression and reduced TG content and lipid droplets in DM-induced 3T3-L1 cells. MiR-182 inhibitor reversed the effect of oe-ELF3 on adipogenic differentiation in 3T3-L1.
UNASSIGNED: ELF3/miR-182/
TSHR axis alleviated Graves\' orbitopathy by inhibiting adipogenic differentiation.