■评估结直肠癌(CRC)中T淋巴细胞亚群分布及双阴性T(DNT)细胞的诊断和预后价值。
■这项回顾性研究比较了114例CRC患者与107例健康对照(HC)的T淋巴细胞亚群和DNT。使用受试者工作特征(ROC)曲线评估DNT和T淋巴细胞亚群的诊断潜力,使用Kaplan-Meier曲线和Cox回归模型评估预后价值.
■CD8+T细胞和DNT细胞的百分比,和癌胚抗原(CEA)的价值,CRC患者明显高于HC患者,但CD4+/CD8+比值下降。采用ROC曲线分析,DNT细胞百分比,CEA,CD4+/CD8+比值均有较好的诊断效能,曲线下面积(AUC)分别为0.865、0.786和0.624。DNT细胞百分比和CEA的组合具有0.905的AUC,其显著高于任何单个生物标志物的AUC(p<0.05)。在单变量分析中,肿瘤淋巴结转移(TNM)临床分期,CD4+/CD8+比值,和DNT细胞百分比与总生存期(OS)显着相关(p<0.05)。在多变量分析中,TNM临床分期(HR=2.37,95%CI:1.15-4.90),CD4+/CD8+比值降低(HR=0.33,95%CI:0.15-0.74),DNT细胞百分比增加(HR=2.29,95%CI:1.11-4.73)是CRC的独立预后因素。
■DNT细胞百分比可作为CRC诊断和预后的评价指标,与血清CEA联合使用时效果更好。
UNASSIGNED: To evaluate the T-lymphocyte subset distribution and the diagnostic and prognosis value of double-negative T (DNT) cells in colorectal cancer (CRC).
UNASSIGNED: This retrospective study compared the T-lymphocyte subsets and DNT of 114 patients with CRC with those of 107 healthy controls (HC). The diagnostic potential of DNT and T-lymphocyte subsets was assessed using the receiver operating characteristic (ROC) curve, and prognostic values were evaluated using the Kaplan-Meier curve and the Cox regression model.
UNASSIGNED: The percentages of CD8+ T cells and DNT cells, and value of carcinoembryonic antigen (CEA), were remarkably higher in patients with CRC than in those with HC, but the ratio of CD4+/CD8+ was decreased. Using ROC curve analysis, DNT cell percentage, CEA, and CD4+/CD8+ ratio all had good diagnostic efficacy, with areas under the curve (AUCs) of 0.865, 0.786 and 0.624, respectively. The combination of DNT cell percentage and CEA had an AUC of 0.905, which was significantly higher than that of any single biomarker (p < 0.05). In univariate analysis, the Tumor Node Metastasis (TNM) clinical stage, CD4+/CD8+ ratio, and DNT cell percentage were significantly associated with overall survival (OS) (p < 0.05). In multivariate analysis, TNM clinical staging (HR = 2.37, 95 % CI: 1.15-4.90), a decreased CD4+/CD8+ ratio (HR = 0.33, 95 % CI: 0.15-0.74), and an increased DNT cell percentage (HR = 2.29, 95 % CI: 1.11-4.73) were independent prognostic factors for CRC.
UNASSIGNED: The percentage of DNT cells may be useful as an evaluation index for CRC diagnosis and prognosis, which was even better when combined with serum CEA.