背景:脂肪组织(AT)炎症易导致肥胖患者的胰岛素抵抗和代谢综合征。
目的:为了研究脂肪细胞大小,AT炎症,全身性炎症,肥胖的代谢和动脉粥样硬化并发症的性别特异性方式。
方法:横断面队列研究。
方法:荷兰大学医院。
方法:302名BMI≥27kg/m2的成年受试者。
方法:我们获得了腹部皮下脂肪活检,以特定性别的方式,AT炎症的几个参数的关联(包括脂肪细胞大小,巨噬细胞含量,冠状结构(CLS),和基因表达)到全身性炎症的生物标志物,白细胞数量和功能,代谢综合征的存在,胰岛素抵抗,颈动脉粥样硬化斑块,用超声波评估。
结果:脂肪细胞大小与代谢综合征和AT巨噬细胞含量与胰岛素抵抗相关。相比之下,没有一个AT参数与颈动脉粥样硬化有关,尽管抗炎IL-37的mRNA表达与较低的内膜中层厚度相关。我们揭示了深刻的性别差异,BMI和脂肪细胞大小之间的关系,仅在男性中介于脂肪细胞大小和代谢综合征之间。此外,只有男性表现出脂肪细胞大小之间的关联,瘦素和MCP-1的AT表达,以及AT巨噬细胞数量),AT炎症(CLS数)和几种循环炎症蛋白之间,包括hsCRP,IL-6
结论:与肥胖的动脉粥样硬化并发症相比,腹部皮下脂肪组织的炎症与代谢有关。在BMI之间的关联上有很大的性别差异,脂肪细胞大小,AT炎症,全身炎症,男人比女人强壮得多。
Adipose tissue (AT) inflammation predisposes to insulin resistance and metabolic syndrome in obesity.
To investigate the association between adipocyte size, AT inflammation, systemic inflammation, and metabolic and atherosclerotic complications of obesity in a sex-specific manner.
Cross-sectional cohort study.
University hospital in the Netherlands.
A total of 302 adult subjects with a body mass index (BMI) ≥ 27 kg/m2.
We obtained subcutaneous abdominal fat biopsies and systematically assessed, in a sex-specific manner, associations of several parameters of AT inflammation (including adipocyte size, macrophage content, crown-like structures, and gene expression) to biomarkers of systemic inflammation, leukocyte number and function, and to the presence of metabolic syndrome, insulin resistance, and carotid atherosclerotic plaques, assessed with ultrasound.
Adipocyte size was associated with metabolic syndrome and AT macrophage content with insulin resistance. In contrast, none of the AT parameters was associated with carotid atherosclerosis, although mRNA expression of the anti-inflammatory IL-37 was associated with a lower intima-media thickness. We revealed profound sex-specific differences, with an association between BMI and adipocyte size, and between adipocyte size and metabolic syndrome in men only. Also, only men showed an association between adipocyte size, AT expression of leptin and MCP-1, and AT macrophage numbers, and between AT inflammation (crown-like structure number) and several circulating inflammatory proteins, including high specificity C-reactive protein, and IL-6.
Inflammation in abdominal subcutaneous adipose tissue is more related to the metabolic than the atherosclerotic complications of obesity, and there are profound sex-specific differences in the association between BMI, adipocyte size, AT inflammation, and systemic inflammation, which are much stronger in men than women.