DNA聚合酶是使用DNA作为模板合成DNA的酶的超家族。它们对于核酸代谢以及DNA复制和修复是必需的。现代生物技术和分子诊断在分析核酸时严重依赖于DNA聚合酶。在各种发现的DNA聚合酶中,Bst聚合酶,来自嗜热脂肪土芽孢杆菌的DNA聚合酶I的大片段,是最常用的一种,但不如Taq聚合酶研究得很好。Bst聚合酶在合成过程中取代上游DNA链的能力,加上其适度的热稳定性,为几种等温DNA扩增方法提供了基础,包括灯,WGA,RCA,和许多其他人。Bst聚合酶是定义基于等温扩增的诊断测试系统的鲁棒性和分析特性的关键组件之一。这里,我们概述了Bst聚合酶的生化和结构特征,并提供了有关其突变类似物的信息。
DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair. Modern biotechnology and molecular diagnostics rely heavily on DNA polymerases in analyzing nucleic acids. Among a variety of discovered DNA polymerases, Bst polymerase, a large fragment of DNA polymerase I from Geobacillus stearothermophilus, is one of the most commonly used but is not as well studied as Taq polymerase. The ability of Bst polymerase to displace an upstream DNA strand during synthesis, coupled with its moderate thermal stability, has provided the basis for several isothermal DNA amplification methods, including LAMP, WGA, RCA, and many others. Bst polymerase is one of the key components defining the robustness and analytical characteristics of diagnostic test systems based on isothermal amplification. Here, we present an overview of the biochemical and structural features of Bst polymerase and provide information on its mutated analogs.