比较干燥综合征(SS)与健康受试者(HS)的口腔微生物群。
从SS患者(n=57)和年龄和性别匹配的HS(n=53)的近颊侧牙齿表面收集牙龈上和龈下生物膜样品。使用口腔刷采集未刺激的唾液和8个口腔组织样品。记录龋齿和牙周测量。所有牙龈上样本和24个SS和28个HS牙龈下样本的亚组,以及32个SS和11个HS唾液和口腔组织样本,使用棋盘DNA-DNA杂交分析了41种细菌的含量。确定每个样品位点的每个物种的DNA探针计数的平均水平(×105±SEM)和百分比,并在2个临床组中的受试者中进行平均。Kruskal-Wallis测试,调整多重比较和聚类分析,用于软组织和微生物分析,Mann-Whitney检验用于比较龋齿和牙周测量。
与HS(44.1±6.8)组相比,SS(13.3±0.7)的牙龈上样品中的平均(×105±SEM)总DNA探针计数显着降低(P<0.001)。在牙龈上样本中,细小静脉菌,长春核梭杆菌,与HS组相比,SS中痤疮丙酸杆菌的平均(×105±SEM)和平均(±SEM)百分比DNA探针计数均显着升高(P<0.001)。在SS的龈下样本中,与HS相比,V.parvula差异显着(P<0.05)。SS的特征在于高水平的紫色和低水平的橙色和红色复合物。口腔组织和唾液的聚类分析表明,SS患者和HS组的平均微生物谱分别聚类。SS组主动根龋(P<0.003)和附着丧失显著高于HS组(P<0.029)。
这些发现表明唾液是口腔内生物膜的主要控制因素。细小弧菌可能是干燥综合征的独特微生物生物标志物。
唾液功能减退中Sjögren综合征的微生物组显示处于压力下并减少。小静脉菌可能是干燥综合征的生物标志物。
To compare the oral microbiota of Sjögren\'s syndrome (SS) with that of healthy subjects (HS).
Supragingival and subgingival biofilm samples were collected from the mesial-buccal tooth surfaces of SS patients (n = 57) and age- and sex-matched HS (n = 53). Unstimulated saliva and 8 oral tissue samples were taken using a buccal brush. Caries and periodontal measures were recorded. All supragingival samples and a subgroup of 24 SS and 28 HS subgingival samples, as well as 32 SS and 11 HS saliva and oral tissue samples, were analyzed for their content of 41 bacterial species using checkerboard DNA-DNA hybridization. Mean levels (×105 ± SEM) and percentage of DNA probe counts of each species were determined for each sample site and averaged within subjects in the 2 clinical groups. Kruskal-Wallis tests, adjusting for multiple comparisons and cluster analysis, were used for soft tissue and microbial analysis, and the Mann-Whitney test was used to compare caries and periodontal measures.
Mean (×105 ± SEM) total DNA probe counts in supragingival samples were significantly lower (P < 0.001) in the SS (13.3 ± .7) compared to the HS (44.1 ± 6.8) group. In supragingival samples, Veillonella parvula, Fusobacterium nucleatum ss vincenti, and Propionibacterium acnes were markedly elevated in the SS compared to the HS group in both mean (×105 ± SEM) and mean (± SEM) percentage DNA probe counts (P < 0.001). In subgingival samples of SS, V. parvula was significantly different compared to HS (P < 0.05). SS was characterized by high levels of purple and low levels of orange and red complexes. Cluster analysis of oral tissues and saliva demonstrated that the mean microbial profiles for SS patients and the HS group clustered separately. Active root caries (P < 0.003) and attachment loss were significantly higher (P < 0.029) in the SS group compared to the HS group.
These findings indicate that saliva is a major controlling factor of intraoral biofilm. V. parvula may be a unique microbial biomarker for Sjögren\'s syndrome.
The microbiome characterized for Sjögren\'s syndrome in salivary hypofunction is shown to be under stress and reduced. Veillonella parvula can be a possible identification of a biomarker for Sjögren\'s syndrome.