背景:本研究旨在阐明蒙脱石粉对II期压疮小鼠伤口愈合的治疗作用和潜在机制,从而为其在此类溃疡的治疗中的临床应用提供了坚实的基础。
方法:将60只8周龄无特定病原体的雄性BALB/c小鼠随机分为三组:模型组(采用磁铁压法诱导II期压疮,伤口用0.9%氯化钠溶液浸泡纱布包扎),治疗组(其中,II期压疮模型诱导后,用蒙脱石粉均匀治疗伤口),和对照组(其中磁铁被放置在相同的位置,而不施加磁性压力)。通过光学显微镜评估皮肤组织病理学。利用Image-ProPlus软件对不同间隔内的伤口愈合进展进行定量。通过苏木精和曙红(H&E)染色检查伤口的组织病理学改变。使用链霉亲和素-过氧化物酶方法分析伤口组织中生长因子蛋白的表达。此外,血管内皮生长因子(VEGF)的水平,I型和III型胶原蛋白(COL-I,COL-III)蛋白通过蛋白质印迹进行定量,通过酶联免疫吸附测定小鼠血清炎症介质浓度,采用紫外可见分光光度法测定创面组织氧化应激标志物水平。
结果:治疗组血清白细胞介素-1β水平明显降低,白细胞介素-6和肿瘤坏死因子-α,与模型组相比,白细胞介素-4水平升高(p<0.05)。此外,转化生长因子-β1,碱性成纤维细胞生长因子,表皮生长因子,VEGF,COL-I,治疗组创面组织中COL-III蛋白明显高于模型组(p<0.05)。伤口组织中超氧化物歧化酶和谷胱甘肽过氧化物酶的水平较高,与模型组相比,治疗组丙二醛水平降低(p<0.05)。
结论:蒙脱石粉剂可促进模型小鼠II期压疮的伤口愈合,并提高愈合率。其作用机制可能与减轻伤口炎症有关,减少氧化应激损伤,促进血管生成,增强生长因子和胶原蛋白的合成。
BACKGROUND: This study aims to elucidate the therapeutic effects and underlying mechanisms of montmorillonite powder on wound healing in mice with Stage II pressure ulcers, thereby providing a robust foundation for its clinical application in the treatment of such ulcers.
METHODS: Sixty 8-week-old specific pathogen-free male BALB/c mice were randomly allocated into three groups: a model group (where Stage II pressure ulcers were induced using the magnet pressure method and the wounds were dressed with gauze soaked in 0.9% sodium chloride solution), a treatment group (where, following the induction of Stage II pressure ulcer models, wounds were uniformly treated with montmorillonite powder), and a control group (where magnets were placed in the same location without exerting magnetic pressure). Skin histopathology was assessed via light microscopy. Wound healing progress over various intervals was quantified utilizing Image-Pro Plus software. Histopathological alterations in the wounds were examined through hematoxylin and eosin (H&E) staining. The expression of growth factor proteins within the wound tissue was analyzed using the streptavidin-peroxidase method. Furthermore, the levels of vascular endothelial growth factor (VEGF), collagen types I and III (COL-I, COL-III) proteins were quantified via Western blotting, serum concentrations of inflammatory mediators in mice were determined by enzyme-linked immunosorbent assay, and the levels of oxidative stress markers in wound tissues were measured using UV-visible spectrophotometry.
RESULTS: The treatment group exhibited significantly reduced serum levels of interleukin-1β, interleukin-6, and tumor necrosis factor-alpha, and elevated levels of interleukin-4 compared to the model group (p < 0.05). Additionally, the expression of transforming growth factor-beta1, basic fibroblast growth factor, epidermal growth factor, VEGF, COL-I, and COL-III proteins in wound tissues was significantly higher in the treatment group than in the model group (p < 0.05). Levels of superoxide dismutase and glutathione peroxidase in wound tissues were higher, and levels of malondialdehyde were lower in the treatment group compared to the model group (p < 0.05).
CONCLUSIONS: Montmorillonite powder facilitates wound healing and augments the healing rate of Stage II pressure ulcers in model mice. Its mechanism of action is likely associated with mitigating wound inflammation, reducing oxidative stress damage, promoting angiogenesis, and enhancing the synthesis of growth factors and collagen.