目的:评估受母系遗传减数分裂非整倍体影响的胚胎的早期胚胎发育潜力。
方法:这种观察,描述性研究包括151例患者的930个卵母细胞,通过结合形态学评估和极体诊断的遗传结果进行回顾性分析。
结果:在检查的930个卵母细胞中,566(60.9%)被测试为非整倍体。卵裂期的发育潜力不受三体或单体的影响(69.6%vs.77.1%,p=0.75)。然而,与单体相比,三体明显更容易导致高质量的卵裂期胚胎(20%与17.6%,p=<0.01)。优质胚泡比非整倍体更可能是整倍体(52.4%vs.47.6%,p=0.032)。此外,与整倍体胚胎相比,明显更多的非整倍体胚胎导致发育停滞(15.3%vs.6.7%,p=0.003)。总的来说,囊胚期胚胎中三体和单体的频率没有显着差异。(28.3%与28.2%;p=0.81)。与早期的发育阶段相比,三体和单体的分布在优质胚泡中没有差异(8.3%vs.5.3%,p=0.32)。然而,某些染色体异常显示发育为最高胚泡的可能性更高.这些包括单体2、5、8、10、16、17、20、21和22以及三体2、4、5、8、9、10、11、12、13、16、17、18和20。
结论:减数分裂诱导的母体非整倍体对早期胚胎发育有不同的影响。虽然在胚泡中没有观察到单体和三体之间的发育潜力差异,卵裂期质量受染色体非整倍体的显著影响。
OBJECTIVE: To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies.
METHODS: This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the genetic results from polar body diagnosis.
RESULTS: Of 930 oocytes examined, 566 (60.9%) were tested aneuploid. Developmental potential until cleavage stage was not affected by trisomies or monosomies (69.6% vs. 77.1%, p = 0.75). However, trisomies significantly more often resulted in top quality cleavage stage embryos compared to monosomies (20% vs. 17.6%, p = < 0.01). Top quality blastocysts were more likely to be euploid than aneuploid (52.4% vs. 47.6%, p = 0.032). Additionally, significantly more aneuploid embryos resulted in developmental arrest compared to euploid embryos (15.3% vs. 6.7%, p = 0.003). Overall, there was no significant difference in the frequency of trisomies and monosomies in blastocyst stage embryos. (28.3% vs. 28.2%; p = 0.81). In contrast to earlier developmental stages, distribution of trisomies and monosomies did not differ in top quality blastocysts (8.3% vs. 5.3%, p = 0.32). However, certain chromosomal abnormalities showed a higher potential to develop into a top-rated blastocyst. These included monosomies 2, 5, 8, 10, 16, 17, 20, 21, and 22 and trisomies 2, 4, 5, 8, 9, 10, 11, 12, 13, 16, 17, 18 and 20.
CONCLUSIONS: Meiotically induced maternal aneuploidies have different effects on early embryonic development. While no difference in developmental potential between monosomies and trisomies could be observed in blastocysts, cleavage stage quality was significantly affected by chromosomal aneuploidies.