PDF(Pubmed)
Abstract：
OBJECTIVE: This study aims to achieve the methodological improvement of rescue IVM by predicting germinal vesicle breakdown (GVBD) and optimizing the timing of ICSI.
METHODS: Time lapse analysis was performed retrospectively to evaluated the relationship between the presence of AC around the nucleoli and GVBD. To find the optimal timing of ICSI, the time from the initiation of the first polar body extrusion to ICSI were measured, and the rates of fertilization at each point were calculated.
RESULTS: The GVBD rate of GV stage oocytes with AC around the nucleoli was significantly higher than that of GV stage oocytes without AC. The GV stage oocytes required more time for nuclear maturation after polar body extrusion than MI oocytes, with GV stage oocytes taking 400-600 min from polar body extrusion to the optimal timing of ICSI, while the MI stage oocytes took 200-400 min. The GV stage oocytes resulted in the birth of healthy babies with the appropriate timing of ICSI.
CONCLUSIONS: It was found that GV stage oocytes with AC around nucleoli can initiate GVBD and reach the MII stage with a high rate, and that GV stage oocytes required more time than MI stage oocytes to reach the optimal timing of ICSI. Considering these factors, ART laboratories may employ immature GV stage oocytes in routine ART procedures rather than discarding them.
METHODS: Time lapse analysis was performed retrospectively to evaluated the relationship between the presence of AC around the nucleoli and GVBD. To find the optimal timing of ICSI, the time from the initiation of the first polar body extrusion to ICSI were measured, and the rates of fertilization at each point were calculated.
RESULTS: The GVBD rate of GV stage oocytes with AC around the nucleoli was significantly higher than that of GV stage oocytes without AC. The GV stage oocytes required more time for nuclear maturation after polar body extrusion than MI oocytes, with GV stage oocytes taking 400-600 min from polar body extrusion to the optimal timing of ICSI, while the MI stage oocytes took 200-400 min. The GV stage oocytes resulted in the birth of healthy babies with the appropriate timing of ICSI.
CONCLUSIONS: It was found that GV stage oocytes with AC around nucleoli can initiate GVBD and reach the MII stage with a high rate, and that GV stage oocytes required more time than MI stage oocytes to reach the optimal timing of ICSI. Considering these factors, ART laboratories may employ immature GV stage oocytes in routine ART procedures rather than discarding them.
摘要：
目的：本研究旨在通过预测生发囊泡破裂（GVBD）和优化ICSI的时机来实现抢救IVM的方法学改进。
方法：回顾性分析了时差分析，以评估核仁周围AC的存在与GVBD之间的关系。为了找到ICSI的最佳时机，测量从第一极体挤出开始到ICSI的时间，计算各点的受精率。
结果：核仁周围有AC的GV期卵母细胞的GVBD率明显高于无AC的GV期卵母细胞。GV期卵母细胞在极体挤压后需要比MI卵母细胞更多的时间进行核成熟，GV期卵母细胞从极体挤压到ICSI的最佳时机需要400-600分钟，而MI阶段卵母细胞需要200-400分钟。GV期卵母细胞在适当的ICSI时机导致健康婴儿的出生。
结论：发现核仁周围有AC的GV期卵母细胞可以启动GVBD并达到MII期，GV期卵母细胞比MI期卵母细胞需要更多的时间才能达到ICSI的最佳时机。考虑到这些因素,ART实验室可以在常规ART程序中使用未成熟的GV阶段卵母细胞，而不是丢弃它们。
方法：回顾性分析了时差分析，以评估核仁周围AC的存在与GVBD之间的关系。为了找到ICSI的最佳时机，测量从第一极体挤出开始到ICSI的时间，计算各点的受精率。
结果：核仁周围有AC的GV期卵母细胞的GVBD率明显高于无AC的GV期卵母细胞。GV期卵母细胞在极体挤压后需要比MI卵母细胞更多的时间进行核成熟，GV期卵母细胞从极体挤压到ICSI的最佳时机需要400-600分钟，而MI阶段卵母细胞需要200-400分钟。GV期卵母细胞在适当的ICSI时机导致健康婴儿的出生。
结论：发现核仁周围有AC的GV期卵母细胞可以启动GVBD并达到MII期，GV期卵母细胞比MI期卵母细胞需要更多的时间才能达到ICSI的最佳时机。考虑到这些因素,ART实验室可以在常规ART程序中使用未成熟的GV阶段卵母细胞，而不是丢弃它们。
