■光疗,以其高选择性而闻名,副作用少,可控性强,和联合治疗的协同增强,广泛用于治疗宫颈癌等疾病。
■在这项研究中,以中空介孔二氧化锰为载体构建带正电荷,聚(烯丙基胺盐酸盐)-修饰的纳米颗粒(NPs)。通过添加磷酸氢盐离子,使NP有效负载光敏剂吲哚菁绿(ICG),以产生抗衡离子聚集效应。进行HeLa细胞膜包封以获得最终的M-HMnO2@ICGNP。在这个结构中,HMnO2载体在肿瘤微环境中响应性降解释放ICG,自生O2对ICG介导的光动力疗法(PDT)敏感,并消耗GSH以扩大氧化应激治疗效果[化学动力学疗法(CDT)+PDT]。在肿瘤组织中积累的ICG通过单激光照射发挥协同PDT/光热治疗(PTT)作用,提高效率和减少副作用。细胞膜封装增加了纳米药物在肿瘤组织中的积累并赋予免疫逃避能力。此外,PTT引起的局部高温可以增强CDT。NP的这些性质使得能够完全实现PTT/PDT/CDT和靶向作用。
■Mn2+可以作为磁共振成像剂指导治疗,和ICG可用于光热和荧光成像。静脉注射后,M-HMnO2@ICG在小鼠肿瘤部位有效积累;体内激光治疗的最佳时机可以通过近红外荧光来验证,磁共振,和光热成像。在近红外光条件下,M-HMnO2@ICGNPs在治疗组中具有最佳的抗肿瘤效果。表现出良好的生物相容性。
■在这项研究中,我们设计了一种改善缺氧的纳米仿生递送系统,对肿瘤微环境的反应,并有效地加载ICG。它为宫颈癌的协同光疗和CDT治疗提供了一种新的经济便捷的策略。
UNASSIGNED: Phototherapy, known for its high selectivity, few side effects, strong controllability, and synergistic enhancement of combined treatments, is widely used in treating diseases like cervical cancer.
UNASSIGNED: In this study, hollow mesoporous manganese dioxide was used as a carrier to construct positively charged, poly(allylamine hydrochloride)-modified nanoparticles (NPs). The NP was efficiently loaded with the photosensitizer indocyanine green (ICG) via the addition of hydrogen phosphate ions to produce a counterion aggregation effect. HeLa cell membrane encapsulation was performed to achieve the final M-HMnO2@ICG NP. In this structure, the HMnO2 carrier responsively degrades to release ICG in the tumor microenvironment, self-generates O2 for sensitization to ICG-mediated photodynamic therapy (PDT), and consumes GSH to expand the oxidative stress therapeutic effect [chemodynamic therapy (CDT) + PDT]. The ICG accumulated in tumor tissues exerts a synergistic PDT/photothermal therapy (PTT) effect through single laser irradiation, improving efficiency and reducing side effects. The cell membrane encapsulation increases nanomedicine accumulation in tumor tissues and confers an immune evasion ability. In addition, high local temperatures induced by PTT can enhance CDT. These properties of the NP enable full achievement of PTT/PDT/CDT and targeted effects.
UNASSIGNED: Mn2+ can serve as a magnetic resonance imaging agent to guide therapy, and ICG can be used for photothermal and fluorescence imaging. After its intravenous injection, M-HMnO2@ICG accumulated effectively at mouse tumor sites; the optimal timing of in-vivo laser treatment could be verified by near-infrared fluorescence, magnetic resonance, and photothermal imaging. The M-HMnO2@ICG NPs had the best antitumor effects among treatment groups under near-infrared light conditions, and showed good biocompatibility.
UNASSIGNED: In this study, we designed a nano-biomimetic delivery system that improves hypoxia, responds to the tumor microenvironment, and efficiently loads ICG. It provides a new economical and convenient strategy for synergistic phototherapy and CDT for cervical cancer.