背景:急性弛缓性麻痹(AFP)是一种具有多种病因的复杂临床综合征。如果未经治疗,AFP可能由于呼吸肌衰竭而导致死亡。滴答麻痹,这是AFP的一种非感染性神经综合征,发生在勾号附件之后,充血,注射蜱唾液毒素.蜱麻痹没有专门的诊断测试,和死亡率随着明确诊断的延迟而增加。尽管进行了蜱唾液的代谢组学研究,缺乏对受蜱麻痹影响的宿主进行代谢组学评估的研究。
目标:因此,本研究的目的是使用基于NMR的代谢组学研究血根病导致蜱麻痹的犬血清样本中的代谢组学变化,并鉴定潜在的诊断/预后标志物.
方法:40只狗感染了血门,临床发现与AFP和确诊的蜱麻痹诊断相符,组成了瘫痪组。十只健康的狗,被接纳用于疫苗接种和/或检查目的,组成了控制组。确认蜱麻痹后,病史,疫苗接种和营养状况,记录了所有狗的体表面积和估计的蜱数。体格检查包括体温,心脏和呼吸频率,毛细血管补充时间评估和改进的格拉斯哥昏迷量表计算。从所有狗的静脉血样本中提取血清样本,并准备用于NMR分析。并进行基于NMR的代谢组学鉴定和定量。
结果:本研究的基于NMR的血清代谢组学显示出明显的上调/下调表达,提出了一个有希望的途径。此外,据观察,能量代谢,特别是肝功能受损的狗与蜱麻痹,不仅呼吸系统受到影响,肾脏也受到影响。
结论:结论是,本方法可能有助于更好地理解蜱麻痹导致AFP的病理机制。
BACKGROUND: Acute flaccid paralysis (AFP) is a complex clinical syndrome with various aetiologies. If untreated, AFP may lead to death due to failure of respiratory muscles. Tick paralysis, which is a noninfectious neurologic syndrome of AFP, occurs following tick attachment, engorgement, and injection of tick saliva toxins. There is no specific diagnostic test for tick paralysis, and mortality increases as definitive diagnosis is delayed. Although metabolomic investigation of tick saliva was conducted, there is a lack of research on metabolomic evaluation of hosts affected by tick paralysis.
OBJECTIVE: Thus, the aim of this study is to investigate metabolomic changes in serum samples of dogs with tick paralysis due to Rhipicephalus sanguineus using NMR-based metabolomics and to identify potential diagnostic/prognostic markers.
METHODS: Forty dogs infested with R. sanguineus, with clinical findings compatible with AFP and with a confirmed tick paralysis diagnosis ex juvantibus, constituted the Paralysis Group. Ten healthy dogs, which were admitted either for vaccination and/or check-up purposes, constituted the Control Group. After the confirmation tick paralysis, medical history, vaccination and nutritional status, body surface area and estimated tick numbers of all the dogs were noted. Physical examination included body temperature, heart and respiratory rate, capillary refill time evaluation and Modified Glasgow Coma Scale calculation. Serum samples were extracted from venous blood samples of all the dogs and were prepared for NMR analysis, and NMR-based metabolomics identification and quantification were performed.
RESULTS: NMR-based serum metabolomics of the present study revealed distinct up/down-regulated expressions, presenting a promising avenue. Moreover, it was observed that energy metabolism and especially liver functions were impaired in dogs with tick paralysis, and not only the respiratory system but also the kidneys were affected.
CONCLUSIONS: It was concluded that the present approach may help to better understand the pathological mechanisms developing in cases of AFP due to tick paralysis.