目的是检查由聚(L-乳酸)-共聚(ε-己内酯)制成的支架的效率,胶原蛋白(COL),聚苯胺(PANI),并在大鼠模型中富含脂肪干细胞(ASCs)作为神经导管。对P(LLA-CL)-COL-PANI支架进行优化,并电纺丝成管状结构。收获来自10只Lewis大鼠的脂肪组织用于ASC培养。共有28只近交雄性Lewis大鼠接受了坐骨神经横切和10毫米神经干碎片切除。A组,神经间隙保持不变;在B组中,切除的躯干用作自体移植物;在C组中,神经残端用P(LLA-CL)-COL-PANI导管固定;在D组中,用ASC富集P(LLA-CL)-COL-PANI导管。经过6个月的观察,处死大鼠。收集腓肠肌和坐骨神经的重量,组织学分析,和神经纤维计数分析。A组表现为肌肉晚期萎缩,和每次干预(B,C,D)防止肌肉质量下降(p<0.0001);然而,ASCs添加降低了效率与自体移植(p<0.05)。神经纤维计数显示,在使用导管的组中观察到的神经纤维密度具有优异的效果(D与Bp<0.0001,Cvs.Bp<0.001)。具有ASC的P(LLA-CL)-COL-PANI导管在通过减少肌肉萎缩来管理神经间隙方面显示出有希望的结果。
The aim was to examine the efficiency of a scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen (COL), polyaniline (PANI), and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model. P(LLA-CL)-COL-PANI scaffold was optimized and electrospun into a tubular-shaped structure. Adipose tissue from 10 Lewis rats was harvested for ASCs culture. A total of 28 inbred male Lewis rats underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In Group A, the nerve gap remained untouched; in Group B, an excised trunk was used as an autograft; in Group C, nerve stumps were secured with P(LLA-CL)-COL-PANI conduit; in Group D, P(LLA-CL)-COL-PANI conduit was enriched with ASCs. After 6 months of observation, rats were sacrificed. Gastrocnemius muscles and sciatic nerves were harvested for weight, histology analysis, and nerve fiber count analyses. Group A showed advanced atrophy of the muscle, and each intervention (B, C, D) prevented muscle mass decrease (p < 0.0001); however, ASCs addition decreased efficiency vs. autograft (p < 0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the groups with the use of conduit (D vs. B p < 0.0001, C vs. B p < 0.001). P(LLA-CL)-COL-PANI conduits with ASCs showed promising results in managing nerve gap by decreasing muscle atrophy.