当前的研究使用VITEK2紧凑型自动微生物鉴定系统评估了从清洁中游尿液样品中获得的557个大肠杆菌分离株的抗生素敏感性和超广谱β-内酰胺酶(ESBL)产量。使用不同种类的药物来确定最小抑制浓度(MIC)。在我们的研究中,50.45%的分离物是ESBL生产者。女性(77.4%)的UTI发病率高于男性(22.6%)。分离株对萘啶酸等抗生素的耐药性百分比很高(89.59%),氨苄青霉素(75.76%),替卡西林(73.43%),头孢菌素(67.68%),头孢克肟(65.17%),环丙沙星(58.35%)和头孢曲松(56.37%)。观察到分离株对磷霉素(98.03%)和呋喃妥因(91.002%)等药物类别的敏感性增加。在分离物中,395(70.91%)根据观察到的针对三种或更多种抗生素的耐药模式被归类为多药耐药生物。一种对氟喹诺酮类药物有抗性的分离株,青霉素,青霉素和β-内酰胺酶抑制剂,氨基糖苷类,对第三代头孢菌素和碳青霉烯类进行全基因组测序(WGS).WGS数据显示该分离株是一种高风险克隆ST410,其中含有抗微生物抗性基因(blaTEM-1B,blaCTX-M-15,blaNDM-5,aac(3)-IId,arma,gyrA(p.S83L),gyrA(p.D87N))赋予对β-内酰胺的抗性,头孢菌素,碳青霉烯,氨基糖苷类和氟喹诺酮类抗生素。使用BacWGSTdb进行核心基因组MLST以评估基因组序列的整体系统发育关系。
■在线版本包含补充材料,可在10.1007/s12088-023-01125-1获得。
The current study evaluates antibiotic susceptibility and Extended Spectrum β-Lactamase (ESBL) production of 557 Escherichia coli isolates obtained from clean catch midstream urine samples using VITEK 2 compact automated microbial identification system. Different classes of drugs were used to determine the Minimum inhibitory concentration (MIC). In our study, 50.45% of isolates were ESBL producers. There is a higher incidence of UTI in females (77.4%) than in males (22.6%). The isolates reveal a high percentage of resistance to antibiotics like nalidixic acid (89.59%), ampicillin (75.76%), ticarcillin (73.43%), cefalotin (67.68%), cefixime (65.17%), ciprofloxacin (58.35%) and ceftriaxone (56.37%). An increased susceptibility pattern was observed for the isolates against drug classes like fosfomycin (98.03%) and nitrofurantoin (91.02%). Among the isolates, 395 (70.91%) were classified as Multidrug-resistant organisms based on the resistance pattern observed against three or more classes of antibiotics. One of the isolates resistant to fluoroquinolones, penicillins, penicillins along with β-lactamase inhibitor, aminoglycosides, third-generation cephalosporins and carbapenems was subjected to Whole genome sequencing (WGS). WGS data revealed the isolate to be a high-risk clone ST410, which contains antimicrobial-resistance genes (blaTEM-1B, blaCTX-M-15, blaNDM-5, aac(3)-IId, armA, gyrA(p.S83L), gyrA(p.D87N)) conferring resistance to β-lactam, cephalosporins, carbapenem, aminoglycoside and fluoroquinolone class of antibiotics. The core genome MLST was carried out using BacWGSTdb to assess the global phylogenetic relationship of the genome sequence.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12088-023-01125-1.