氧化应激被认为是体外成熟(IVM)的主要挑战之一,包括IVF和胚胎发育效果较差。通过生物相容性纳米粒子(NP)减少自由基是开发IVM的最有前途的方法之一。我们研究了绿色和化学合成的氧化铁纳米颗粒(IONP)与枣树花粉(DPP)的水提取物对与IVM过程相关的卵母细胞参数的比较效果。为此,IONP通过化学方法(Ch-IONP)和绿色方法(使用DPP的G-IONP)合成并表征。通过JC1和Hoechst染色评价Ch-IONP和G-IONP组的成熟卵母细胞质量,膜联蛋白V-FITC-碘化丙啶,2\',7\'-二氯荧光素二乙酸酯,和二氢乙啶染色与对照组相比。最终,成熟的卵母细胞受精,促进胚泡(BL),并在体外进行评价。与对照组和G-IONP组相比,Ch-IONP处理组产生更多的过氧化氢和氧自由基。与Ch-IONP组相比,G-IONP组和对照组的受精率显着提高。最后,G-IONP和对照组在2PN中表现出显著的增加,2细胞,4细胞,8细胞,压实桑兰(CM),与Ch-IONP组相比,BL率。IONP的绿色合成可以降低IVM过程中化学IONP的毒性。可以得出结论,用DPP化合物包裹的G-IONP具有在IVM培养基中防止外源活性氧(ROS)产生的潜力,这对卵母细胞成熟和受精效率有至关重要的影响。
Oxidative stress is considered one of the main challenges for in vitro maturation (IVM) and makes assisted reproductive technology (ART), including IVF and embryonic development less effective. Reducing free radicals via biocompatible nanoparticles (NPs) is one of the most promising approaches for developing IVM. We investigated the comparative effect of green and chemically synthesized iron oxide nanoparticles (IONPs) with an aqueous extract of date palm pollen (DPP) on oocyte parameters related to the IVM process. To this end, IONPs were synthesized by chemical (Ch-IONPs) and green methods (G-IONPs using DPP) and characterized. The mature oocyte quality of the Ch-IONPs and G-IONPs groups was evaluated by JC1 and Hoechst staining, Annexin V-FITC-Propidium Iodide, 2\', 7\'-dichlorofluorescein diacetate, and dihydroethidium staining compared to the control group. Eventually, the mature oocytes were fertilized, promoted to blastocysts (BL), and evaluated in vitro. Compared with the control and G-IONPs groups, the Ch-IONPs-treated group produced more hydrogen peroxide and oxygen radicals. Compared with the Ch-IONPs group, the fertilization rate in the G-IONPs and control groups increased significantly. Finally, the G-IONPs and control groups exhibited a significant increase in the 2PN, 2-cell, 4-cell, 8-cell, compacted morula (CM), and BL rates compared with the Ch-IONPs group. Green synthesis of IONPs can reduce the toxicity of chemical IONPs during the IVM process. It can be concluded that G-IONPs encased with DPP compounds have the potential to protect against exogenous reactive oxygen species (ROS) production in an IVM medium, which can have a crucial effect on oocyte maturation and fertilization efficiency.