目的:本研究的目的是通过口腔黏膜微核试验评估接受口服人类免疫缺陷病毒暴露前预防的个体的细胞遗传学变化。
方法:本研究由37个人组成,其中17人是暴露前预防组,20人是对照组。每个载玻片总共分析了2,000个细胞以确定微核,双核,核芽,和细胞毒性参数:固缩,核溶解,和karyorrhexis(KR),以双盲的方式。在该设置中还评估了修复指数。
结果:在致突变性参数中,暴露前预防组显示微核频率增加(p=0.0001),双核(p=0.001),和核芽(p=0.07)。关于细胞毒性参数,有统计学差异(p≤0.05)的核裂频率增加(p=0.001)。此外,暴露前预防组的修复系统效率下降。
结论:这些结果表明,接受暴露前预防使用的个体在口腔粘膜细胞中具有基因和细胞毒性。
OBJECTIVE: The objective of this study was to evaluate cytogenetic changes in individuals submitted to oral human immunodeficiency virus pre-exposure prophylaxis use through the micronucleus test in oral mucosa.
METHODS: This study consisted of 37 individuals, of whom 17 comprised the pre-exposure prophylaxis group and 20 comprised the control group. A total of 2,000 cells per slide were analyzed for the determination of micronuclei, binucleation, nuclear buds, and cytotoxicity parameters: pyknosis, karyolysis, and karyorrhexis (KR), in a double-blind manner. The repair index was also evaluated in this setting.
RESULTS: In the mutagenicity parameters, the pre-exposure prophylaxis group showed increased frequencies of micronuclei (p=0.0001), binucleation (p=0.001), and nuclear buds (p=0.07). Regarding the cytotoxicity parameters, there was an increase with a statistical difference (p≤0.05) in the karyorrhexis frequency (p=0.001). Additionally, the repair system efficiency decreased in the pre-exposure prophylaxis group.
CONCLUSIONS: These results indicate that individuals undergoing pre-exposure prophylaxis use have geno- and cytotoxicity in oral mucosal cells.