■本研究旨在开发一种针对心肌肌钙蛋白I(cTnI)的高灵敏度和选择性单链DNA(ssDNA)适体,急性心肌梗死(AMI)的重要生物标志物。目的是在丝网印刷碳电极(SPCE)上使用钴镍金属有机骨架(CoNi-MOF)的复合材料制造新型适体电化学传感器,利用复合材料的大表面积和优异的导电性以及适体对cTnI的高亲和力。
■在SPCE上使用CoNi-MOF复合材料制造了适体电化学传感器,并表征了其性能。他们进行了电化学测量,以评估传感器在检测cTnI方面的性能。传感器的稳定性,再现性,和电催化活性进行了评价。
■传感器在5-75pg/mL的浓度范围内证明了cTnI的线性检测,低检测限(LOD)为13.2pM。在cTnI检测中观察到显著的稳定性和重现性。该传感器表现出优异的电催化活性,能够准确定量各种解决方案中的cTnI水平。
■这项研究为开发可靠的,成本效益高,和临床应用易于部署的cTnI传感器。该传感器在不同浓度范围内检测cTnI的多功能性突出了其在不同临床环境中的潜在效用。特别是早期检测和监测心脏状况。
UNASSIGNED: This study aimed to develop a highly sensitive and selective single-stranded DNA (ssDNA) aptamer targeting cardiac troponin I (cTnI), a crucial biomarker for acute myocardial infarction (AMI). The objective was to fabricate a novel aptamer electrochemical sensor using a composite material of cobalt-nickel metal-organic framework (CoNi-MOF) on screen-printed carbon electrodes (SPCE), leveraging the composite\'s large surface area and excellent electrical conductivity alongside the aptamer\'s high affinity for cTnI.
UNASSIGNED: The aptamer electrochemical sensor was fabricated using the CoNi-MOF composite on SPCE and characterized its properties. They conducted electrochemical measurements to assess the sensor\'s performance in detecting cTnI. The sensor\'s stability, reproducibility, and electro-catalytic activity were evaluated.
UNASSIGNED: The sensor demonstrated linear detection of cTnI over a concentration range of 5-75 pg/mL, with a low limit of detection (LOD) of 13.2 pM. Remarkable stability and reproducibility were observed in cTnI detection. The sensor exhibited exceptional electro-catalytic activity, enabling accurate quantification of cTnI levels in various solutions.
UNASSIGNED: This research presents a significant advancement towards the development of reliable, cost-effective, and easily deployable cTnI sensors for clinical applications. The sensor\'s versatility in detecting cTnI across different concentration ranges highlights its potential utility in diverse clinical settings, particularly for early detection and monitoring of cardiac conditions.