伤口易于感染,这可能对患者的生命是致命的。抗生素的使用对于控制伤口中的细菌感染至关重要,但是长期使用高剂量的抗生素可能会导致细菌耐药性,甚至产生超级细菌。因此,制定针对性的抗菌治疗策略和减少抗生素的使用是当务之急.在这项研究中,通过Zn2+的自组装合成了一种用于治疗抑菌感染的多功能纳米药物递送系统(Cef-rhEGF@ZIF-8@ConA),头孢拉定(Cef)和重组人表皮生长因子(rhEGF),然后与伴刀豆球蛋白(ConA)缀合,其经历pH响应性降解以释放药物。首先,ConA能与细菌特异性结合,抑制Zn2+离子的快速释放,从而达到长效抗菌效果。Cef通过抑制细菌膜蛋白的合成发挥其抗菌作用。最后,从Zn-金属-有机骨架(MOF)释放的Zn2离子通过增强细菌细胞膜的渗透性而表现出抑菌特性。此外,rhEGF上调血管生成相关基因,从而促进血管生成,上皮再生和伤口愈合过程。结果表明,Cef-rhEGF@ZIF-8@ConA具有良好的生物相容性,对金黄色葡萄球菌和大肠杆菌的抗菌效果分别为99.61%和99.75%,分别。这些纳米材料可以抑制炎症细胞因子的释放,促进抗炎细胞因子的释放,同时还刺激成纤维细胞的增殖以促进伤口愈合。一起来看,Cef-rhEGF@ZIF-8@ConA纳米系统是抑菌感染和伤口愈合的临床治疗的优秀候选者。
Wounds are prone to infection which may be fatal to the life of the patient. The use of antibiotics is essential for managing bacterial infections in wounds, but the long-term use of high doses of antibiotics may lead to bacterial drug resistance and even to creation of superbacteria. Therefore, the development of targeted antimicrobial treatment strategies and the reduction in antibiotic usage are of utmost urgency. In this study, a multifunctional nanodrug delivery system (Cef-rhEGF@ZIF-8@ConA) for the treatment of bacteriostatic infection was synthesized through self-assembly of Zn2+, cefradine (Cef) and recombinant human epidermal growth factor (rhEGF), then conjugated with concanavalin (ConA), which undergoes pH-responsive degradation to release the drugs. First, ConA can specifically combine with bacteria and inhibit the rapid release of Zn2+ ions, thus achieving a long-acting antibacterial effect. Cef exerts its antibacterial effect by inhibiting the synthesis of bacterial membrane proteins. Finally, Zn2+ ions released from the Zn-metal-organic framework (MOF) demonstrate bacteriostatic properties by enhancing the permeability of the bacterial cell membrane. Furthermore, rhEGF upregulates angiogenesis-associated genes, thereby promoting angiogenesis, re-epithelialization and wound healing processes. The results showed that Cef-rhEGF@ZIF-8@ConA has good biocompatibility, with antibacterial efficacy against Staphylococcus aureus and Escherichia coli of 99.61 % and 99.75 %, respectively. These nanomaterials can inhibit the release of inflammatory cytokines and promote the release of anti-inflammatory cytokines, while also stimulating the proliferation of fibroblasts to facilitate wound healing. Taken together, the Cef-rhEGF@ZIF-8@ConA nanosystem is an excellent candidate in clinical therapeutics for bacteriostatic infection and wound healing.