香蕉(Musaspp。)是全球消费最广泛的水果。枯萎病,由尖孢镰刀菌引起。立方体(Foc),是一种对香蕉生产具有高度威胁的疾病。对Foc的抗性基因存在于野生Musa基因型中,例如Musaacuminata亚种。Burmannicoidesvar.加尔各答4.虽然实时PCR(RT-qPCR)适用于准确分析参与宿主防御反应的途径中的基因表达,在特定生物胁迫条件和宿主组织类型下稳定表达的参考基因对于样品变异的正常化是必需的。在这种情况下,潜在宿主参考基因ACT1、APT、EF1α,GAPDH,αTUB,RAN,在加尔各答4(抗性)和Musasp。的根组织的总RNA样品中评估了UBIQ1,UBIQ2,βTUB1,βTUB3,L2和ACTA1。与Foc亚热带种族4(STR4)相互作用时提取的品种Prata-anã(易感)。使用算法geNorm计算表达式稳定性,NormFinder和BestKeeper。βTUB3和L2在加尔各答4中最稳定,而ACTA1和GAPDH在Prata-anã中最稳定。这些用于分析Musa-FocSTR4病理系统中基因表达调节的参考基因对于增进对这种重要病原体的宿主防御反应的理解至关重要。
Banana (
Musa spp.) is the most widely consumed fruit globally. Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is a highly threatening disease to banana production. Resistance genes to Foc exist in wild
Musa genotypes such as
Musa acuminata subsp. burmannicoides var. Calcutta 4. Whilst real-time PCR (RT-qPCR) is appropriate for accurate analysis of gene expression in pathways involved in host defence responses, reference genes with stable expression under specific biotic stress conditions and host tissue types are necessary for normalization of sample variation. In this context, the stability in potential host reference genes ACT1, APT, EF1α, GAPDH, αTUB, RAN, UBIQ1, UBIQ2, βTUB1, βTUB3, L2 and ACTA1 was evaluated in total RNA samples from root tissues in Calcutta 4 (resistant) and
Musa sp. cultivar Prata-anã (susceptible) extracted during interaction with Foc subtropical race 4 (STR4). Expression stability was calculated using the algorithms geNorm, NormFinder and BestKeeper. βTUB3 and L2 were identified as the most stable in Calcutta 4, with ACTA1 and GAPDH the most stable in Prata-anã. These reference genes for analysis of gene expression modulation in the
Musa-Foc STR4 pathosystem are fundamental for advancing understanding of host defence responses to this important pathogen.