背景:病原体相关蛋白(PR)在植物防御中至关重要,对抗不同的生物和非生物胁迫。虽然多个基因家族有助于香蕉对尖孢镰刀菌fsp。立方体(Foc),假孢子菌,还有Pratylenchus咖啡,PR-1基因在防御中的意义至关重要.
方法:三个PR-1基因,在不同的生物胁迫下上调,从Foc的抗性和易感品种中克隆,P.eumusae,还有咖啡。分子表征,系统发育分析,并进行了与FocTR4CP基因的对接研究。
结果:通过转录组学和实时研究,来自Musaspp的三个PR-1基因(Ma02_g15050,Ma02_g15060和Ma04_g34800)。已确定。当暴露于Foc时,这些基因在抗性品种中表现出显著的上调,P.eumusae,还有咖啡。从Foc种族1和TR4,P.eumusae的抗性和易感品种中成功克隆了这些基因,还有咖啡。在PR-1基因中观察到不同的特征,第1组和第2组是酸性的信号肽,和组3是碱性的,没有信号肽。所有克隆的PR-1蛋白都属于CAP超家族(PF00188)。系统发育分析揭示了酸性PR-1蛋白的聚类模式,KEGG矫形学显示与生命通路相关,包括MAPK信号,植物激素信号转导,和植物-病原体相互作用。二级和三级结构分析证实了所研究物种的序列保守性。对接研究探索了FocTR4的cerato-platanin(CP)基因与香蕉的Ma02_g15060之间的相互作用,提示PR-1抗真菌活性通过直接相互作用的潜在障碍。
结论:这些发现强调了克隆的PR-1基因在香蕉植物抵抗广谱生物胁迫的防御机制中的关键作用。这些基因,尤其是第1和第2组的那些,有望成为开发耐逆性香蕉品种的候选人。该研究为香蕉防御策略的分子方面提供了有价值的见解,强调PR-1基因在增强香蕉抗逆性方面的潜在应用。
BACKGROUND: Pathogen-related proteins (PR) are pivotal in plant defense, combating diverse biotic and abiotic stresses. While multiple gene families contribute to banana resistance against Fusarium oxysporum f sp. cubense (Foc), Pseudocercospora eumusae, and Pratylenchus coffeae, the significance of PR-1 genes in defense is paramount.
METHODS: Three PR-1 genes, up-regulated under diverse biotic stresses, were cloned from both resistant and susceptible cultivars of Foc, P. eumusae, and P. coffeae. Molecular characterization, phylogenetic analysis, and docking studies with the Foc TR4 CP gene were conducted.
RESULTS: Through transcriptomic and real-time studies, three PR-1 genes (Ma02_g15050, Ma02_g15060, and Ma04_g34800) from
Musa spp. were identified. These genes exhibited significant up-regulation in resistant cultivars when exposed to Foc, P. eumusae, and P. coffeae. Cloning of these genes was successfully performed from both resistant and susceptible cultivars of Foc race 1 and TR4, P. eumusae, and P. coffeae. Distinct characteristics were observed among the PR-1 genes, with groups 1 and 2 being acidic with signal peptides, and group 3 being basic without signal peptides. All cloned PR-1 proteins belonged to the CAP superfamily (PF00188). Phylogenetic analysis revealed clustering patterns for acidic PR-1 proteins, and KEGG orthology showed associations with vital pathways, including MAPK signaling, plant hormone signal transduction, and plant-pathogen interaction. Secondary and tertiary structure analyses confirmed sequence conservation across studied species. Docking studies explored interactions between the cerato-platanin (CP) gene from Foc TR4 and Ma02_g15060 from banana, suggesting the potential hindrance of PR-1 antifungal activity through direct interaction.
CONCLUSIONS: The findings underscore the crucial role of cloned PR-1 genes in banana plant defense mechanisms against a broad spectrum of biotic stresses. These genes, especially those in groups 1 and 2, hold promise as candidates for developing stress-tolerant banana cultivars. The
study provides valuable insights into the molecular aspects of banana defense strategies, emphasizing the potential applications of PR-1 genes in enhancing banana resilience.