背景:扁平苔藓(LPP)是一种慢性淋巴细胞性皮肤病,表现为进行性瘢痕性脱发。LPP的诊断是根据组织病理学检查,虽然并不总是确定的。当前的研究评估了非侵入性原子力显微镜(AFM)头发检查在检测健康和患病头发之间的形态差异方面的有效性。
方法:这里,从10名LPP患者的病变皮肤中收集了3至5根毛发,并使用AFM在9个位置进行了检查。在9个地点中的每个地点至少拍摄了4张图像。采用公制测量和公制(长度,宽度,和尺度台阶高度)和形态特征(尺度的条纹和光滑表面,内膜和皮质的存在,尺度边缘的形状,划痕,点蚀,裂缝,小球,和波浪形边缘)与健康对照的头发进行比较。此外,病变毛发上病理过程的区域,描述了头发纤维发生的非自然分层。
结果:在LPP头发的初始部分中,划痕数量存在统计学上的显着差异,在整个长度的波浪形边缘的强度测试的头发,以及头发中部点蚀的鳞片数量。此外,在LPP组中,从从根部3.5cm开始,一直到头发的自由端,发现具有条纹表面的鳞屑的数量具有统计学意义。其他形态变化,如皮质的存在,小球,椭圆形凹痕,和杆状大原纤维元素也进行了评估,然而,没有提供详细的结果,由于这些形态变化的数量差异没有显着差异。
结论:本出版物概述了处女之间的区别,健康的白种人头发,和LPP患者的头发。本研究结果可用于与LPP相关的进一步研究和工作。这是使用AFM表征LPP患者毛发的首次尝试。
BACKGROUND: Lichen planopilaris (LPP) is a chronic lymphocytic skin disease manifested by progressive scarring alopecia. The diagnosis of LPP is made based on histopathological examination, although it is not always definite. The current study evaluates the effectiveness of non-invasive atomic force microscopy (AFM) hair examination in detecting morphological differences between healthy and diseased hair.
METHODS: Here, three to five hairs from lesional skin of 10 LPP patients were collected and examined at nine locations using AFM. At least four images were taken at each of the nine sites. Metric measurements were taken and metric (length, width, and scale step height) and morphological features (striated and smooth surface of scales, the presence of endocuticle and cortex, shape of scales edges, scratches, pitting, cracks, globules, and wavy edge) were compared with hair from healthy controls. In addition, areas on diseased hair where the process of pathological, unnatural delamination of the hair fiber occurs are described.
RESULTS: There was a statistically significant difference in the number of scratches in the initial sections of the LPP hair, in the intensity of wavy edges along the entire length of the tested hair, and in the number of scales with pitting in the middle section of the hair. In addition, a statistically significant higher number of scales with striated surface was found in LPP group starting at 3.5 cm from the root continuing towards the free end of the hair. Other morphological changes such as presence of cortex, globules, oval indentations, and rod-like macrofibrillar elements were also assessed, however, detailed results are not presented, as the differences shown in the number of these morphological changes were not significantly different.
CONCLUSIONS: This publication outlines the differences between virgin, healthy Caucasian hair, and the hair of LPP patients. The results of this study can be used for further research and work related to LPP. This is the first attempt to characterize the hair of LPP patients using AFM.