传染性胰腺坏死(IPN)是一种高度传染性疾病,在幼鱼中引起高死亡率。由于没有有效的方法来治疗IPNV,早期诊断和预防在防治疾病中起着重要作用。不同类型的IPNV疫苗(非活性,活,重组,DNA,等)是从当地分离株生产的,并已在发达国家使用。在土耳其,没有针对IPNV的商业许可疫苗。由于这个原因,土耳其需要IPNV疫苗。尝试从属于sp血清型的分离物中生产重组VP2亚单位疫苗(IPNV-VP2)和灭活的全颗粒病毒疫苗(IPNV-WPV)。为此,在RTG-2细胞系中产生病毒,并通过使用具有限制酶的引物进行RT-PCR扩增。将整个VP2基因克隆到质粒载体中,用大肠杆菌表达系统表达VP2。进行了一项试验,以确定IPNV-VP2和IPNV-WPV在虹鳟鱼中的免疫能力。根据SN50分析,IPNV-WPV比IPNV-VP2疫苗更快地刺激免疫应答。此外,存活的相对百分比(RPS)在接种IPNV-WPV的鱼中检测为79%,在接种IPNV-VP2的鱼中检测为70%。因此,我们可以说,IPNV-VP2的重组疫苗几乎可以抵抗IPNV感染以及非活性疫苗。
Infectious pancreatic necrosis (IPN) is a highly contagious disease causing high mortality in juvenile trouts. Since there is no effective way to treatment against IPNV, early diagnosis and prevention play an important role in combating the disease. The different types of IPNV vaccines (inactive, live, recombinant, DNA, etc) have been produced from local isolates and have been used in developed countries. In Turkey, there is no commercial licensed vaccines against IPNV. Due to this reason,
IPNV vaccine is needed in Turkey. The production of recombinant VP2 subunit vaccine (IPNV-VP2) and inactivated whole particle virus vaccine (IPNV-WPV) were attempted from selected isolate belong to sp serotype. For this purpose; the virus was produced in RTG-2 cell line and RT-PCR amplification was performed by using primers with restriction enzymes. The whole VP2 gene was cloned into a plasmid vector and VP2 was expressed by using E. coli expression system. A trial was conducted to determine the immunity ability of
IPNV-VP2 and
IPNV-WPV in rainbow trout. According to the SN50 assay, the
IPNV-WPV stimulates immune response faster than the
IPNV-VP2 vaccine. Besides, the relative percent of Survive (RPS) was detected as 79% in fish vaccinated with IPNV-WPV and 70% in fish vaccinated with
IPNV-VP2. Thus, we can say that the recombinant vaccine of
IPNV-VP2 is almost protected against
IPNV infection as well as the inactive vaccine.