这项研究的目的是双重的:(1)抗原(Ag)的制备和评估三种抗原。粗体细胞抗原(CSAg),排泄分泌抗原(ESAg)和部分纯化的抗原(即P1Ag,P2Ag和P3Ag)区分IgE,IgG,IgG1-4和IgM用于人类颌骨病诊断;(2)选择的ELISA用于跟踪伊维菌素(IVM)和阿苯达唑(ABZ)治疗的患者的储存血清。
不同的抗原被分析的抗体的颌口病病例,其他寄生虫感染和健康对照使用间接ELISA来区分IgE,IgG,IgG1-4和IgM。然后,突出的抗原和免疫球蛋白用于阿苯达唑或伊维菌素治疗的颌口病病例的抗体预测。
所有评估ELISA的灵敏度:IgM-,IgG-,IgG1-和IgG4-ELISA,是100%。用CSAg和P3Ag的IgM-ELISA表现出最高的99%的特异性。用P2Ag的IgG-ELISA导致92.3%的最高特异性。P2Ag和P3Ag的IgG1-ELISA显示出优异的结果,具有100%的特异性。最后,P2Ag评估ABZ和IVM随访病例的IgG1。抗体IgG1水平下降主要在第9个月的两种治疗中发现,长期随访超过12个月。从每两名接受治疗的患者中提取出一条Gnathostoma蠕虫。
使用针对P2Ag和P3Ag的IgG1-ELISA给出了具有100%灵敏度和特异性的优异结果。这些测试可以替代免疫印迹治疗颌骨口病。在大多数情况下,IgG1下降至少9个月,因此,长期治疗应超过1年。
The aims of the study were two-fold: (1) antigen (Ag) preparation and evaluation of three antigens of Gnathostoma spinigerum infective larvae (GsL3), crude somatic antigen (CSAg), excretory-secretory antigen (ESAg) and partially purified antigens (namely P1Ag, P2Ag and P3Ag) to differentiate IgE, IgG, IgG1-4 and IgM for human
gnathostomiasis diagnosis; and (2) application of the selected ELISA for following up stored sera of patients treated with ivermectin (IVM) and albendazole (ABZ).
Different antigens were analysed by antibodies of
gnathostomiasis cases, other parasite infections and healthy controls using indirect ELISA to differentiate IgE, IgG, IgG1-4 and IgM. Then, prominent antigen and immunoglobulin were used in antibody predictions of
gnathostomiasis cases treated with albendazole or ivermectin.
Sensitivity of all evaluated ELISAs: IgM-, IgG-, IgG1- and IgG4-ELISA, was 100%. IgM-ELISA with CSAg and P3Ag exhibited the highest specificity of 99%. IgG-ELISA with P2Ag resulted in the highest specificity of 92.3%. IgG1-ELISA with P2Ag and P3Ag showed excellent results with 100% specificity. Finally, P2Ag evaluated IgG1 of the followed-up cases with ABZ and IVM. Decreasing antibody IgG1 levels were mostly found in both treatments at Month 9 and long follow-up was over 12 months. A Gnathostoma worm was extracted from each two treated patients.
Using IgG1-ELISA against P2Ag and P3Ag gave excellent results with 100% sensitivity and specificity. These tests can be an alternative to immunoblotting for
gnathostomiasis. IgG1 decreased at least 9 months in most cases, so long-term treatment should be performed over 1 year.