Rad51是DNA修复相关基因,据报道与多种癌症有关。然而,它与肝癌中的免疫浸润的联系仍然未知。因此,需要对Rad51在肝细胞癌(HCC)中的作用和活性进行更多研究。国际癌症基因组联盟(ICGC)用于鉴定DNA修复基因Rad51,并已被证明在HCC患者中过表达。我们绘制了Kapan-Meier曲线,表明Rad51高表达的患者预后不良。通过分析病人数据,我们发现Rad51在HCC中的高表达与临床分期有关,病理T分期,grade,和年龄。使用多变量cox模型发现Rad51是HCC患者的独立预后因素。此外,发现Rad51的表达与免疫细胞的浸润有关(B细胞,CD4+T细胞,CD8+T细胞,中性粒细胞,巨噬细胞,和树突状细胞),并且与HCC中免疫细胞标志物的表达密切相关。通过对Rad51,GO和KEGG富集分析的差异共表达基因(DCG)的分析,表明Rad51的表达水平可能与神经活性配体-受体相互作用有关,细胞周期,DNA复制,同源重组,卵母细胞减数分裂,和范可尼贫血途径。这些发现表明Rad51是肝癌患者预后的有价值的生物标志物,其表达与免疫浸润具有显着相关性。缩写:HCC:肝细胞癌;ICGC:国际癌症基因组联盟TCGA:癌症基因组图谱;TIMER:肿瘤免疫评估资源;CAF:癌症相关成纤维细胞;GEPIA:基因表达谱交互式分析;GSEA:基因集富集分析;OS:总生存期;PFS:无进展生存期;RFS:无复发生存期;DSS:疾病特异性生存期。部分相关系数:部分相关系数;HPA:人类蛋白质图谱;GO:基因本体论;KEGG:京都基因和基因组百科全书;CAF:癌症相关成纤维细胞;DCG:差异共表达基因。
Rad51, a DNA-repair-related gene, has been reported to be involved in multiple cancers. However, its link with immune infiltration in liver cancer still unknown. Therefore, more research into the roles and activities of Rad51 in hepatocellular carcinoma (HCC) is required. The International Cancer Genome Consortium (ICGC) was used to identify the DNA repair gene Rad51, and has been proved to be overexpressed in HCC patients. We plotted the Kapan-Meier curve, demonstrating that patients with high expression of Rad51 have a poor prognosis. By analyzing the patient data, we discovered that high expression of Rad51 in HCC is linked to clinical stage, pathological T stage, grade, and age. Rad51 was found to be an independent prognostic factor for HCC patients using the multivariate cox model. Moreover, Rad51 expression was found to be associated with the infiltration of immune cells (B cells, CD4 + T cells, CD8 + T cells, neutrophils, macrophages, and dendritic cells) and was intimately linked to the expression of immune cell markers in HCC. Through the analysis of differentially coexpressed genes (DCGs) of Rad51, GO and KEGG enrichment analyses suggested that the expression level of Rad51 might be relevant to neuroactive ligand-receptor interactions, the cell cycle, DNA replication, homologous recombination, oocyte meiosis, and the Fanconi anemia pathway. These findings indicated that Rad51 is a valuable biomarker for the prognosis of patients with liver cancer and that its expression has a significant correlation with immune infiltrations.Abbreviations: HCC: hepatocellular carcinoma; ICGC: International Cancer Genome Consortium TCGA: The Cancer Genome Atlas; TIMER: Tumor Immune Estimation Resource; CAF: Cancer-associated fibroblast; GEPIA: Gene Expression Profiling Interactive Analysis; GSEA: Gene set enrichment analysis; OS: overall survival; PFS: progression-free survival; RFS: relapse-free survival; DSS: disease-specific survival. Partial cor: partial correlation coefficient; HPA: Human Protein Atlas; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; CAF: Cancer-associated fibroblast; DCGs: differentially co-expressed genes.