参与代谢调节的基因,脂肪组织沉积,炎症,食欲-饱腹感轴可能在胎儿发育中起重要作用,并可能引起永久性代谢变化和脂肪积累。在这项研究中,我们调查了:(1)超重/肥胖和正常体重孕妇的母体和脐带血中肥胖相关基因的表达;(2)母体和脐带血中肥胖相关基因表达之间的关联;(3)每种母体和脐带血中基因表达与新生儿肥胖的关联。
根据孕前BMI,从Araraquara队列研究中选择了25名超重/肥胖孕妇和32名正常体重孕妇。母体和脐带血LEPR基因表达,STAT3、PPARG、TLR4,IL-6,IL-10,FTO,MC4R,TNF-α,通过相对实时PCR定量研究NFκB。通过空气置换体积描记术评估新生儿的身体成分。母体和脐带血基因表达与新生儿肥胖标志物之间的关联(体重,BMI,和脂肪质量%)通过控制产妇年龄的线性回归模型进行探索,孕前BMI,孕妇妊娠期体重增加,胎龄,新生儿性
TLR4、NFκB、和TNF-a表达,和较低的IL-6表达,超重/肥胖孕妇及其新生儿与正常体重妇女及其新生儿相比(p<0.001)。母体PPARG基因表达与新生儿的体重和脂肪质量百分比有关,脐带血IL-10表达与BMI和脂肪质量%相关,控制混杂因素。
据我们所知,这是首次评估母体和脐带血基因表达与新生儿肥胖标志物关系的研究。我们的结果为母体和脐带血基因表达的贡献提供了证据,特别是母体PPARG和TLR4表达,和脐带血IL-10的表达-新生儿体重,BMI,和脂肪质量%。
Genes involved in the regulation of metabolism, adipose tissue deposition, inflammation, and the appetite-satiety axis may play an important role in fetal development, and possibly induce permanent metabolic changes and fat accumulation. In this study we investigated: (1) obesity-related gene expression in maternal and cord blood of overweight/obese and normal-weight pregnant women; (2) associations between obesity-related gene expression in maternal and cord blood; and (3) associations of gene expression in each of maternal and cord blood with newborn adiposity.
Twenty-five overweight/obese and 32 normal-weight pregnant women were selected from the Araraquara Cohort Study according to their pre-pregnancy BMI. Maternal and cord blood gene expression of LEPR, STAT3, PPARG, TLR4, IL-6, IL-10, FTO, MC4R, TNF-α, and NFκB were investigated by relative real-time PCR quantification. The body composition of the newborns was assessed by air displacement plethysmography. Associations between maternal and cord blood gene expression and markers of newborn adiposity (weight, BMI, and fat mass%) were explored by linear regression models controlling for maternal age, pre-pregnancy BMI, maternal gestational weight gain, gestational age, and newborn sex.
There was higher TLR4, NFκB, and TNF-a expression, and lower IL-6 expression, in overweight/obese pregnant women and their respective newborns compared with normal-weight women and their newborns (p < 0.001). Maternal PPARG gene expression was associated with both weight and fat mass % of the newborns, and cord blood IL-10 expression was associated with BMI and fat mass %, controlling for confounders.
To our knowledge, this is the first study to evaluate the relationship of maternal and cord blood gene expression with adiposity markers of the newborn. Our results provide evidence for the contribution of maternal and cord blood gene expression-particularly maternal PPARG and TLR4 expression, and cord blood IL-10 expression-to newborn weight, BMI, and fat mass %.