目的:比较使用现成的Vacutainer管产生乙二胺四乙酸(EDTA)溶液的3种方法,以治疗带状角膜病变。
方法:所有3种方案均使用市售的涂有K2EDTA的Vacutainer采血管。使用渗透压计来测量和比较使用3种不同方案产生的EDTA的浓度。测量并比较制备溶液所需的时间以评估其日常临床使用的效率。此外,对于方法1,测量获得的EDTA溶液的体积。然后将最有希望的临床使用方案用于治疗一系列患者。
结果:方法1、2和3的平均摩尔渗透压浓度分别为532、285和422(ANOVAP<0.01,所有Tukey均诚实地显着差异P<0.01)。对于各自的混合物,平均浓度为65、35和52mg/mL,创建溶液的平均时间为189、38和83秒(ANOVAP<0.01,所有Tukey均诚实显着差异P<0.01)。最有希望的,方法3,在一系列5例6眼患者中发现从角膜基质中去除钙是安全有效的。与方法1相比,它还产生了25%的临床使用解决方案。
结论:方法3使用含有18mgK2-EDTA的单个10-mLVacutainer管具有EDTA有效浓度的最佳平衡,准备时间,方法简单,与以前发表的方法1和2相比。它还产生更大的最终溶液体积。
OBJECTIVE: To compare 3 methods for creating ethylenediaminetetraacetic acid (EDTA) solution using readily available Vacutainer tubes for the treatment of band keratopathy.
METHODS: All 3 protocols used commercially available Vacutainer blood collection tubes coated with K2EDTA. An osmometer was used to measure and compare the concentration of EDTA created using 3 different protocols. The time required for preparation of the solution was measured and compared to evaluate its efficiency for everyday clinical use. In addition, volume of EDTA solution obtained was measured for method 1. The most promising protocol for clinical use was then used for treatment of a series of patients.
RESULTS: Average osmolarity was 532, 285, and 422 for methods 1, 2, and 3, respectively (ANOVA P < 0.01, all Tukey honestly significant difference P < 0.01). For the respective mixtures, average concentration was 65, 35, and 52 mg/mL, and average time to create solution was 189, 38, and 83 seconds (ANOVA P < 0.01, all Tukey honestly significant difference P < 0.01). The most promising, method 3, was found to be safe and effective in removing calcium from the corneal stroma in a series of 5 patients with 6 eyes treated. It also yielded 25% more solution for clinical use than method 1.
CONCLUSIONS: Method 3 using a single 10-mL Vacutainer tube with 18 mg of K2-EDTA had the best balance of effective concentration of EDTA, time to preparation, and simplicity of methodology, when compared with previously published methods 1 and 2. It also yielded a greater final volume of solution.