PDF(Pubmed)
Abstract：
Oncology treatments cause infertility, and ovarian tissue cryopreservation and transplantation (OTCT) is the only option for fertility preservation in prepubertal girls with cancer. However, OTCT is associated with massive follicle loss. Here, we aimed to determine the effect of supplementation of slow freezing and vitrification media with BAPTA-AM and melatonin alone and in combination on ovarian tissue viability, reactive oxygen species (ROS) levels, total antioxidant capacity (TAC), and follicular morphology and viability. Our results indicated that BAPTA-AM and melatonin can significantly improve ovarian tissue viability and the TAC/ROS ratio and reduce ROS generation in frozen-thawed ovarian tissues in slow freezing and vitrification procedures. BAPTA-AM was also found to be less effective on TAC compared to melatonin in vitrified ovarian tissue. While supplementation of slow freezing and vitrification media with BAPTA-AM and/or melatonin could increase the percentage of morphologically intact follicles in cryopreserved ovarian tissues, the differences were not significant. In conclusion, supplementation of cryopreservation media with BAPTA-AM or melatonin improved the outcome of ovarian tissue cryopreservation in both vitrification and slow freezing methods. Our data provide some insight into the importance of modulating redox balance and intracellular Ca2+ levels during ovarian tissue cryopreservation to optimize the current cryopreservation methods.
摘要：
肿瘤治疗会导致不孕,卵巢组织冷冻保存和移植（OTCT）是保留青春期前癌症女孩生育能力的唯一选择。然而,OTCT与大量卵泡损失有关。这里,我们旨在确定单独补充BAPTA-AM和褪黑素的缓慢冷冻和玻璃化培养基对卵巢组织活力的影响，活性氧（ROS）水平，总抗氧化能力(TAC)，和卵泡形态和活力。我们的结果表明，BAPTA-AM和褪黑激素可以显着提高卵巢组织的活力和TAC/ROS比率，并在缓慢冷冻和玻璃化过程中减少冻融卵巢组织中的ROS生成。还发现BAPTA-AM与玻璃化卵巢组织中的褪黑激素相比对TAC的效果较差。虽然用BAPTA-AM和/或褪黑激素补充缓慢冷冻和玻璃化培养基可以增加冷冻保存的卵巢组织中形态完整卵泡的百分比，差异不显著。总之,补充BAPTA-AM或褪黑素的冷冻保存培养基可改善玻璃化和缓慢冷冻方法中卵巢组织冷冻保存的结果。我们的数据为卵巢组织冷冻保存过程中调节氧化还原平衡和细胞内Ca2水平的重要性提供了一些见解，以优化当前的冷冻保存方法。
