■银环蛇是中国十大毒蛇之一。它的毒液主要是神经毒素。新型抗蛇毒血清药物有待进一步研究和开发。
■本研究以网络药理学为基础,探讨白钱花治疗银环蛇咬伤的分子机制。材料和方法。利用PubChem数据库筛选出潜在活性成分,并将其SDF结构导入SwissTargetPrediction数据库,并获得了白钱花在治疗银环蛇咬伤中的抗毒素作用的目标。利用Cytoscape软件构建了蓝藻活性化合物潜在目标网络和蛋白质相互作用网络,然后使用DAVID进行生物学功能分析和KEGG途径富集分析。
■七个潜在的活性成分(西帕诺苷C,凤梨苷B,tomentolideA,谷甾醇,sarcostin,tomentoxenin,和丹皮酚)和286个药物靶标,包括30个治疗银环蛇毒素毒性的关键目标。活性成分主要作用于PIK3CA,MAPK1,MAP2K1,JAK2,FYN,ACHE,CHRNA7,CHRNA4和CHRNB2,它们通过胆碱能突触和神经营养蛋白信号通路拮抗银环蛇毒素对神经系统的抑制作用。
■西南芥通过多种活性成分对银环蛇咬伤发挥治疗作用,多个目标,和多种途径。本研究结果为蓝藻活性成分的提取及相关抗蛇毒血清试验提供了理论依据。
UNASSIGNED: Bungarus multicinctus is one of the top ten venomous snakes in China. Its venom is mainly neurotoxin-based. Novel antivenom drugs need to be further researched and developed.
UNASSIGNED: This study aimed to explore the molecular mechanism of Cynanchum paniculatum in treating Bungarus multicinctus bites based on network pharmacology. Material and methods. The potential active ingredients of Cynanchum paniculatum were screened and their SDF structures were obtained using the PubChem database and imported into the SwissTargetPrediction database, and targets were obtained for the antitoxin effects of Cynanchum paniculatum in the treatment of Bungarus multicinctus bites. The Cynanchum paniculatum-active compound-potential target network and protein-protein interaction network were constructed by using Cytoscape software, and then biological function analysis and KEGG pathway enrichment analysis were performed using the DAVID.
UNASSIGNED: Seven potential active components (cynapanoside C, cynatratoside B, tomentolide A, sitosterol, sarcostin, tomentogenin, and paeonol) and 286 drug targets were obtained, including 30 key targets for the treatment of bungarotoxin toxicity. The active components mainly acted on PIK3CA, MAPK1, MAP2K1, JAK2, FYN, ACHE, CHRNA7, CHRNA4, and CHRNB2, and they antagonized the inhibitory effect of bungarotoxin on the nervous system through cholinergic synapses and the neurotrophin signaling pathway.
UNASSIGNED: Cynanchum paniculatum exerts a therapeutic effect on Bungarus multicinctus bites through multiple active components, multiple targets, and multiple pathways. The findings provide a theoretical basis for the extraction of active components of Cynanchum paniculatum and for related antivenom experiments.