糖尿病是由于葡萄糖代谢受损而发生的慢性疾病。在高血糖状态,氧化应激和抗氧化酶之间的平衡被破坏,导致氧化损伤和细胞死亡。此外,受损的自噬导致细胞中功能失调的蛋白质和细胞器的储存。因此,自噬的细胞保护功能可能被高葡萄糖条件破坏。Alpha-mangostin(A-MG)是从山竹果果实中纯化的必需黄原酮。α-芒果苷的不同药理益处,包括抗氧化剂,抗肥胖,和抗糖尿病药,被证明了。
■我们评估了A-MG对人脐静脉内皮细胞(HUVEC)中高浓度葡萄糖受损的自噬反应的保护性影响。用各种葡萄糖浓度(5-60mM)和A-MG(1.25-10μM)处理HUVEC三天。然后,HUVEC用60mM的葡萄糖+2.5μM的A-MG处理以测量活力,ROS,没有内容。最后,通过蛋白质印迹评估自噬蛋白,包括LC3,SIRT1和beclin1的水平。
■结果表明,高葡萄糖条件(60mM)降低了HUVEC中的活力并增加了ROS和NO含量。此外,当HUVEC暴露于高浓度的葡萄糖时,LC3,SIRT1和beclin1蛋白水平下降。A-MG逆转了这些有害作用并升高了自噬蛋白水平。
■我们的数据表明,A-MG通过减少ROS和NO的产生以及增加自噬蛋白的表达来保护HUVEC免受高糖条件的影响。
UNASSIGNED: Diabetes is a chronic disorder that occurs as a result of impaired glucose metabolism. In hyperglycaemic states, the balance between oxidative stress and antioxidant enzymes is disrupted leading to oxidative damage and cell death. In addition, impaired autophagy leads to the storage of dysfunctional proteins and cellular organelles in the cell. Hence, the cytoprotective function of autophagy may be disrupted by high glucose conditions. Alpha-mangostin (A-MG) is an essential xanthone purified from the mangosteen fruit. The different pharmacological benefits of alpha-mangostin, including antioxidant, anti-obesity, and antidiabetic, were demonstrated.
UNASSIGNED: We evaluated the protective influence of A-MG on autophagic response impaired by high concentrations of glucose in human umbilical vein endothelial cells (HUVECs). The HUVECs were treated with various glucose concentrations (5-60 mM) and A-MG (1.25-10 μM) for three days. Then, HUVECs were treated with 60 mM of glucose+2.5 μM of A-MG to measure viability, ROS, and NO content. Finally, the levels of autophagic proteins including LC3, SIRT1, and beclin 1 were evaluated by western blot.
UNASSIGNED: The results expressed that high glucose condition (60 mM) decreased viability and increased ROS and NO content in HUVECs. In addition, LC3, SIRT1, and beclin 1 protein levels declined when HUVECs were exposed to the high concentration of glucose. A-MG reversed these detrimental effects and elevated autophagic protein levels.
UNASSIGNED: Our data represent that A-MG protects HUVECs against high glucose conditions by decreasing ROS and NO generation as well as increasing the expression of autophagy proteins.