背景对BACH1功能作用的研究表明,BACH1促进癌症转移并调节转移过程的代谢网络。然而,关于BACH1蛋白在乳腺肿瘤中的表达及其作为乳腺肿瘤患者生物标志物与临床变量的相关性知之甚少.方法使用从患者队列(N=130)分离的乳腺肿瘤组织的组织微阵列(TMA),通过免疫组织化学(IHC)测定法监测BACH1及其靶基因MCT1(由SLC16A1编码)的表达,并对其进行评分以进行进一步分析。我们检查了BACH1(Allredscoretal)或MCT1(Hscoretotal3x2x1x)评分与临床变量之间的关联,包括:乳腺癌亚型,组织类型,肿瘤大小,患者的种族/民族背景,和年龄组。使用Mann-WhitneyU检验(或适当时的非参数Kruskal-Wallis检验)比较各组的数字数据。比例赔率序数逻辑模型用于检查多个协变量。用Spearman相关系数评估变量之间的关联。结果IHC检测到90.76%(N=118/130)和92.30%(N=120/130)的BACH1和MCT1表达,分别,在我们的研究中。对肿瘤大小进行二分法后(小:直径与直径为3-25大:直径27-85毫米),与较小的肿瘤组(3.920[1.693])相比,较大肿瘤组的BACH1表达评分显著较高(p=0.015)(平均值[SD];4.20[1.796])。感兴趣的,我们还观察到,与白人女性(3.02[1.942];N=49)相比,黑人女性(3.971[1.514];N=69)的BACH1评分(p=0.004)显著更高.与mRNA表达分析一致,BACH1在所有亚型中的基底样肿瘤中表达最丰富,特别是黑人女性,而无论种族如何,基底样肿瘤中的MCT1表达评分都要高得多。此外,黑人女性肿瘤的BACH1和MCT1IHC评分呈正相关,尽管它们在白人女性肿瘤中的联系较弱。总的来说,我们没有检测到MCT1IHC得分与种族之间的关联,肿瘤大小,组织类型,或者病人的年龄。结论我们发现BACH1表达与肿瘤大小和基底样亚型密切相关。分别。重要的是,BACH1在黑人女性肿瘤中的表达明显高于白人女性,以及黑人女性乳腺肿瘤的基底样亚型。我们的研究表明,BACH1的表达可以作为一个潜在的种族相关的生物标志物,表明预后不良。
UNASSIGNED: Studies on functional roles of
BACH1 reveal that
BACH1 promotes cancer metastasis and regulates metabolic networks for metastatic processes. However, little is known about
BACH1 protein expression in breast tumors and its relevance to clinical variables as a biomarker for patients with breast tumors.
UNASSIGNED: Using a tissue microarray (TMA) of breast tumor tissues isolated from a patient cohort (N = 130) expression of BACH1 and its target gene MCT1 (encoded by SLC16A1) were monitored by immunohistochemistry (IHC) assays and scored for further analyses. We examined the association between scores of
BACH1 (Allredscoretotal) or MCT1 (Hscoretotal3×2×1x) with clinical variables including: breast cancer subtypes, tissue types, tumor size, patient\'s racial/ethnic background, and age group. Groups were compared using the Mann-Whitney U test (or the non-parametric Kruskal-Wallis test when appropriate) for numerical data. A proportional odds ordinal logistic model was used to examine multiple covariates. Associations between variables were evaluated with the Spearman\'s correlation coefficient.
UNASSIGNED: BACH1 and MCT1 expression were detected in 90.76% (N = 118/130) and 92.30% (N = 120/130) of patients by IHC, respectively, in our study. After dichotomizing tumor size (small: 3-25 in diameter vs. big: 27-85 mm in diameter), BACH1 expression scores were significantly higher (p = 0.015) in the bigger tumor group (mean [SD]; 4.20 [1.796]) compared with the smaller tumor group (3.920 [1.693]). Of interest, we also observed significantly higher BACH1 scores (p = 0.004) in tumors from Black women (3.971 [1.514]; N = 69) compared with those of White women (3.02 [1.942]; N = 49). Consistent with mRNA expression analysis,
BACH1 expression is most abundant in the basal-like tumors among all subtypes, specifically in Black women, whereas MCT1 expression scores are considerably higher in the basal-like tumors regardless of race. In addition, there was a positive association between BACH1 and MCT1 IHC scores in tumors from Black women, although a weak association between them in tumors from White women. In general, we did not detect associations between MCT1 IHC scores and race, tumor size, tissue types, or patient\'s age.
UNASSIGNED: We found strong associations of BACH1 expression with tumor size and the basal-like subtype, respectively. Importantly, BACH1 expresses significantly higher in tumors from Black women than White women, as well as in the basal-like subtype of breast tumors from Black women. Our study suggests that BACH1 expression could serve as a potential race-associated biomarker indicating poor prognosis.