■Tahyna病毒(TAHV),一种正双胞病毒属的虫媒病毒,是人类疾病的原因,在世界范围内研究较少。在这项研究中,从伊蚊中分离出一株新的TAHV菌株。盘锦市收集的蚊子,辽宁省。然而,目前尚不清楚中国TAHV的有效载体。
■对新分离的TAHV的基因组进行了测序,并进行了系统发育分析。白纹伊蚊和淡色库蚊口服感染人工病毒血餐(病毒悬液和小鼠血1:1),在中肠中检测到病毒,子房,蚊子的唾液腺和唾液。然后,传播和传播率,评估了蚊子对病毒的垂直传播和水平传播。
■系统发育分析表明,该病毒与TAHV具有高度相似性,并被命名为TAHVPJ01株。口腔感染病毒血餐后,Ae.白纹伊蚊在所有测试组织中均显示病毒阳性,其外部潜伏期为2天,传播和传播率呈波动增加。而在Cx的唾液中未检测到病毒。pipiens苍白。被传染性Ae咬伤的乳鼠。白纹伊蚊出现明显的神经症状,包括不活动,后腿瘫痪,翻身困难,当脑内病毒滴度达到1.70×105PFU/mL时。此外,在卵中检测到THV,Ae的F1后代的幼虫和成虫。白纹。
■Ae.白纹伊蚊是一种有效的传播THV而Cx的载体。pipienspallens不是。Ae.白纹伊蚊也是垂直传播病毒的宿主,这进一步增加了疫情爆发的风险。本研究对中国病原病毒的监测和指导未来病媒综合控制策略以应对潜在的疫情具有重要的流行病学意义。
UNASSIGNED: Tahyna virus (TAHV), an arbovirus of the genus Orthobunyavirus, is a cause of human diseases and less studied worldwide. In this study, a new strain of TAHV was isolated from Aedes sp. mosquitoes collected in Panjin city, Liaoning province. However, the competent vector of TAHV in
China is still unknown.
UNASSIGNED: The genome of newly isolated TAHV was sequenced and phylogenetic analysis is performed. Aedes albopictus and Culex pipiens pallens were orally infected with artificial virus blood meals (1:1 of virus suspension and mouse blood), the virus was detected in the midgut, ovary, salivary gland and saliva of the mosquitoes. Then, the transmission and dissemination rates, vertical transmission and horizontal transmission of the virus by the mosquitoes were assessed.
UNASSIGNED: Phylogenetic analysis revealed that the virus shared high similarity with TAHV and was named the TAHV PJ01 strain. After oral infection with virus blood meals, Ae. albopictus showed positive for the virus in all tested tissues with an extrinsic incubation period of 2 days and a fluctuating increasement of transmission and dissemination rates. Whereas no virus was detected in the saliva of Cx. pipiens pallens. Suckling mice bitten by infectious Ae. albopictus developed obvious neurological symptoms, including inactivity, hind-leg paralysis and difficulty turning over, when the virus titer reached 1.70×105 PFU/mL in the brain. Moreover, TAHV was detected in the eggs, larvae and adults of F1 offspring of Ae. albopictus.
UNASSIGNED: Ae. albopictus is an efficient vector to transmit TAHV but Cx. pipiens pallens is not. Ae. albopictus is also a reservoir host that transmits the virus vertically, which further increases the risk of outbreaks. This study has important epidemiological implications for the surveillance of pathogenic viruses in
China and guiding comprehensive vector control strategies to counteract potential outbreaks in future.