人眼弓形虫病(OT),由宠物蛔虫Toxocaracanis(NematodaAscaridoidea)引起,是一种世界性的眼部寄生虫感染,对视力构成严重威胁,尤其是学龄儿童。然而,弓形虫在人体内的感染过程和病理机制难以研究。本研究旨在探索感染犬弓形虫的不同啮齿动物的长期眼部表现,揭示幼虫感染后的具体病理机制和迁移途径。我们选择的三种实验动物为C57BL/6小鼠,蒙古沙鼠和棕色挪威大鼠。将小鼠随机分为5组,分别口服1000、2000、4000、8000和10,000个犬齿卵;将沙鼠随机分为4组,分别口服1000、2000、4000和10,000个犬齿卵;将大鼠随机分为3组,分别口服2000、6000和10,000个犬齿卵。密切观察他们的眼部变化并记录至少2个月。我们还摘除了一些动物的眼球以进行病理切片和苏木精-伊红染色。3dpi后(感染后天数),出血性病变,在一些感染的动物中可以观察到视网膜的机械损伤和幼虫的迁移。7dpi时眼部感染和死亡率趋于稳定。幼虫组织,在病理切片中可以观察到结构紊乱和炎症。总之,感染了2000只犬卵的小鼠和感染了1000、2000和4000只犬卵的沙鼠表现出明显的眼部病变和较低的死亡率,可以为长期观察提供依据。
Human ocular toxocariasis (OT), caused by pet roundworm Toxocara canis (Nematoda Ascaridoidea), is a worldwide ocular parasitic infection that poses a severe threat to eyesight, especially in school-aged children. However, the infection process and pathological mechanism of Toxocara are difficult to study in the human body. This study was designed to explore long-term ocular manifestations in different rodents infected with Toxocara canis, uncovering the specific pathological mechanism and migration pathway of larvae after infection. The three types of experimental animals we selected were C57BL/6 mice, Mongolian gerbils and Brown Norway rats. Mice were randomly divided into five groups and infected orally with 1000, 2000, 4000, 8000 and 10,000 T. canis eggs; gerbils were randomly divided into four groups and infected orally with 1000, 2000, 4000 and 10,000 T. canis eggs; rats were randomly divided into three groups and infected orally with 2000, 6000 and 10,000 T. canis eggs. Their ocular changes were closely observed and recorded for at least 2 months. We also enucleated the eyeballs of some animals to perform pathological sectioning and hematoxylin-eosin staining. After 3 dpi (days post-infection), hemorrhagic lesions, mechanical injury of the retina and larval migration could be observed in some infected animals. The ocular infection and mortality rates tended to be stable at 7 dpi. Larval tissue, structure disorder and inflammation could be observed in the pathological sections. In conclusion, the mice infected with 2000 T. canis eggs and gerbils infected with 1000, 2000 and 4000 T. canis eggs showing obvious ocular lesions and lower mortality rates could provide a basis for long-term observation.