目的:研究漆酚作为牙本质交联剂的潜力,促进蚀刻牙本质的再矿化,并防止内源性蛋白酶的激活,导致混合层内的胶原蛋白降解。目标是提高树脂-牙本质界面处的粘结强度和耐久性。
方法:使用乙醇和二甲基亚砜(DMSO)作为溶剂制备不同浓度的漆酚引物。来自健康磨牙的牙本质经过研磨和酸蚀15s,然后应用漆酚底漆1min。在再矿化14天和28天后,通过衰减全反射傅里叶变换红外光谱(ATR-FTIR)对再矿化和再矿化进行评估,显微拉曼光谱,X射线衍射(XRD)原子力显微镜(AFM),维氏硬度,扫描电子显微镜(SEM),和能量X射线色散光谱(EDS)。通过微拉伸粘合强度(μTBS)测试和纳米泄漏评估观察了漆酚底漆作为牙本质粘合剂的总体性能。利用原位酶谱研究了漆酚引物对内源性金属蛋白酶(MMPs)的抑制特性,并测试了引物的细胞毒性。
结果:基于ATR-FTIR,拉曼,XRD,EM-EDS和维氏硬度分析,0.7%-乙醇组显着提高牙本质矿物质含量并改善机械性能最大。预处理显着增加了修复体的μTBS,提高了混合层的稳定性,并在28天后降低纳米渗漏和MMPs活性。
结论:漆酚底漆有助于脱矿质牙本质的再矿化,增强蚀刻牙本质的再矿化,有效提高键合界面的稳定性,在0.7wt%浓度的漆酚底漆下观察到最佳性能。
OBJECTIVE: To investigate urushiol\'s potential as a dentin cross-linking agent, promoting remineralization of etched dentin and preventing activation of endogenous proteases causing collagen degradation within the hybrid layer. The goal is to improve bond strength and durability at the resin-dentin interface.
METHODS: Urushiol primers with varying concentrations were prepared using ethanol and dimethyl sulfoxide (DMSO) as solvents. Dentin from healthy molars underwent grinding and acid etching for 15 s, followed by a 1min application of urushiol primer. After 14 and 28 days of remineralization incubation and remineralization were used to assess by Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR), Micro-Raman spectroscopy, X-ray Diffraction (XRD), Atomic Force Microscopy (AFM), Vickers Hardness, Scanning Electron Microscopy (SEM), and Energy X-ray dispersive spectroscopy (EDS). The overall performance of urushiol primers as dentin adhesives was observed by microtensile bond strength (μTBS) testing and nanoleakage assessment. Investigated the inhibitory properties of the urushiol primers on endogenous metalloproteinases (MMPs) utilizing in situ zymography, and the cytotoxicity of the primers was tested.
RESULTS: Based on ATR-FTIR, Raman, XRD, EM-EDS and Vickers hardness analyses, the 0.7%-Ethanol group significantly enhanced dentin mineral content and improved mechanical properties the most. Pretreatment notably increased the μTBS of restorations, promoted the stability of the mixed layer, and reduced nanoleakage and MMPs activity after 28 days.
CONCLUSIONS: The urushiol primer facilitates remineralization in demineralized dentin, enhancing remineralization in etched dentin, effectively improving the bonding interface stability, with optimal performance observed at a 0.7 wt% concentration of the urushiol primer.