■小核仁RNA(snoRNA)是一组富含细胞核的非编码RNA,指导rRNA的转录后修饰,snRNAs和其他分子。最近的研究表明snoRNAs在肿瘤发生中具有重要作用,可以作为预测肿瘤患者总体生存的预后标志物。
■我们从公共数据库中筛选了122个与生存相关的snoRNA,并最终选择了7个与低级别神经胶质瘤(LGG)患者预后最相关的snoRNA,以建立7个snoRNA预后特征。Further,我们将与神经胶质瘤患者预后相关的临床特征和7-snoRNA预后特征相结合,构建列线图.
■预后模型在验证集和分层分析中均显示出更大的预测能力。富集分析结果表明,这些snoRNAs主要参与转录后过程,如RNA剪接,新陈代谢和修饰。此外,7-snoRNA预后特征与免疫评分和多个免疫检查点分子的表达水平呈正相关。可作为免疫疗法预测的潜在生物标志物。从生物信息学分析的结果来看,我们推断SNORD88C在胶质瘤的发生发展中起主要作用,然后进行体外实验验证。结果表明,SNORD88C可以促进细胞增殖,胶质瘤细胞的侵袭和迁移。
■我们建立了7-snoRNA预后签名和列线图,可用于评估LGG患者的生存率,具有良好的敏感性和特异性。此外,SNORD88C可以促进增殖,胶质瘤细胞的迁移和侵袭与DNA和RNA有关的多种生物学过程。
Small nucleolar RNAs (snoRNAs) are a group of non-coding RNAs enriched in the nucleus which direct post-transcriptional modifications of rRNAs, snRNAs and other molecules. Recent studies have suggested that snoRNAs have a significant role in tumor oncogenesis and can be served as prognostic markers for predicting the overall survival of tumor patients.
We screened 122 survival-related snoRNAs from public databases and eventually selected 7 snoRNAs that were most relevant to the prognosis of lower-grade glioma (LGG) patients for the establishment of the 7-
snoRNA prognostic signature. Further, we combined clinical characteristics related to the prognosis of glioma patients and the 7-
snoRNA prognostic signature to construct a nomogram.
The prognostic model displayed greater predictive power in both validation set and stratification analysis. Results of enrichment analysis revealed that these snoRNAs mainly participated in the post-transcriptional process such as RNA splicing, metabolism and modifications. In addition, 7-
snoRNA prognostic signature were positively correlated with immune scores and expression levels of multiple immune checkpoint molecules, which can be used as potential biomarkers for immunotherapy prediction. From the results of bioinformatics analysis, we inferred that SNORD88C has a major role in the development of glioma, and then performed in vitro experiments to validate it. The results revealed that SNORD88C could promote the proliferation, invasion and migration of glioma cells.
We established a 7-
snoRNA prognostic signature and nomogram that can be applied to evaluate the survival of LGG patients with good sensitivity and specificity. In addition, SNORD88C could promote the proliferation, migration and invasion of glioma cells and is involved in a variety of biological processes related to DNA and RNA.