背景:近年来,由于现代生活中久坐的生活方式和不规律的饮食习惯,直肠脱垂的发病率显著增加。然而,目前缺乏大样本量的中药治疗直肠脱垂的临床研究。因此,这项研究调查了直肠脱垂治疗配方的特点,然后研究了其核心治疗药物的网络药理学,有助于为直肠脱垂患者的治疗及术后护理提供参考。
目的:本研究旨在通过数据挖掘和生物信息学技术探讨中药治疗直肠脱垂核心药物的处方特点及作用机制。
方法:收集南京市中医医院电子病例数据库中2014年1月至2021年9月直肠脱垂患者的诊治资料,使用R挖掘患者信息和处方特征,使用TCMSP和GnenCard数据库筛选核心对药物和疾病药物交叉靶标的活性成分,并使用STRING和Cytoscape构建了蛋白质-蛋白质相互作用(PPI)网络,和基因本体论(GO)和京都基因和基因组百科全书(KEGG)的交叉目标的富集分析使用Metascape和R.
结果:我们发现脱垂容易发生在50岁以上的人,最好是在秋天和冬天。常用的治疗中药包括甘草,当归,黄芪,白术,和陈皮,主要是补虚药,温暖的自然,属于脾经。核心治疗药对是“柴胡-升麻”。柴胡和升麻共有190个共同靶标,药物对和脱垂的71个交叉目标。治疗脱垂的核心药物的主要成分可能是槲皮素,山奈酚,豆甾醇,etc,核心靶标可以是CASP3、AKT1、HIF1A、等。“柴胡-升麻”与疾病交叉目标的GO条目总数为3495个,其中分子功能所占比例最大,主要是癌症的途径,IL-18信号通路,等。KEGG富集途径分析得出168个结果,主要途径是癌症的途径,脂质和动脉粥样硬化,IL-17信号通路,等。结论:本研究采用真实世界的研究方法,运用数据挖掘和生物信息学技术,从中医临床信息中挖掘直肠脱垂的用药规律及其核心药物作用机制。
BACKGROUND: In recent years, the incidence of rectal prolapse has increased significantly due to the sedentary lifestyle and irregular eating habits of modern life. However, there is a lack of clinical studies on the treatment of rectal prolapse with traditional Chinese medicine (TCM) with a large sample size. Therefore, this study investigated the characteristics of rectal prolapse treatment formulas and then studied the network pharmacology of their core therapeutic drugs, which can help to provide a reference for the treatment and postoperative care of rectal prolapse patients.
OBJECTIVE: This study aimed to explore the prescription characteristics and the mechanism of action of core drugs in the treatment of rectal prolapse in Chinese medicine through data mining and bioinformatics techniques.
METHODS: We collected the diagnosis and treatment information of patients with rectal prolapse from January 2014 to September 2021 in the electronic case database of Nanjing Hospital of TCM, mined the patient information and prescription features using R, screened the active ingredients of the core pairs of drugs and disease drug intersection targets using TCMSP and GnenCard databases, and constructed a Protein-protein interaction (PPI) network using STRING and Cytoscape, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the intersecting targets were performed using Metascape and R.
RESULTS: We found that prolapse is easy to occur in people over 50 years old, preferably in autumn and winter. Commonly used therapeutic Chinese medicines include Glycyrrhiza glabra, Radix angelicae sinensis, Radix astragali, Atractylodes macrocephala, and Pericarpium citri reticulatae, which are mostly deficiency tonic medicines, warm in nature, and belong to spleen meridian. The core therapeutic medicinal pair was \"Bupleuri radix-Cimicifugae rhizoma\". There were 190 common targets of Bupleuri radix and Cimicifugae rhizoma, and 71 intersection targets of the drug pair and prolapse. The main components of the core drugs for the treatment of prolapse may be quercetin, kaempferol, Stigmasterol, etc, and the core targets may be CASP3, AKT1, HIF1A, etc. The total number of GO entries for the intersection targets of \"Bupleuri radix-Cimicifugae rhizoma\" and diseases was 3495, among which the molecular functions accounted for the largest proportion, mainly Pathways in cancer, IL-18 signaling pathway, etc. KEGG enriched pathway analysis yielded 168 results, and the major pathways were pathways in cancer, lipid and atherosclerosis, IL-17 signaling pathway, etc. Conclusion: This study adopted real-world research methodology and used data mining and bioinformatics technology to mine the medication law of rectal prolapse and its core drug action mechanism from the clinical information of Chinese medicine.