柑橘汁含氧杂环糖苷配基(OHAs)的概况,它们是值得注意的次级代谢物标记,在柑橘和柚子汁可能是掺假的情况下,用于评估甜橙和葡萄柚汁的真实性。39个已知的OHAs,包括10种甲氧基黄酮,13香豆素,和16种呋喃香豆素,以及13个初步筛选的OHAs,用橙色分析,普通话,葡萄柚和柚子汁使用我们新开发的高分辨率HPLC-UV和荧光检测方法。从158个纯果汁样品中获得定量OHA谱,以使用组学策略建立纯度判别模型。OHA变量的减少表明,三种重要的甲氧基黄酮,即异辛塞素,橘皮素和西能塞素在甜橙汁和橘子汁之间提供了最佳的辨别能力。柚子有两种亚型,沙天友集团和文丹集团,其中果汁应与葡萄柚汁分开比较。五个OHAs,即Meranzin,3,5,6,7,8,3',4\'-七乙氧基黄酮,蛇床子,6\',7\'-epoxybergamottin,和佛手香素被发现可以区分沙田油组的柚子汁和葡萄柚汁;而三个OHAs,即Bergaptol,isometranzin,和6\',7'-二羟基佛手香素能够将柚子汁的文丹组与葡萄柚汁区分开。所建立的偏最小二乘判别分析(PLS-DA)模型能够检测甜橙汁中10%的橘子汁和柚子汁中10%的柚子汁,允许以良好的可靠性快速预筛选过量添加(预测的均方根误差,RMSEP<5%)。
Profiles of citrus juice oxygenated heterocyclic aglycones (OHAs), which are notable marker secondary metabolites, were used to assess the authenticity of sweet orange and grapefruit juices in situations where mandarin and pomelo juices might be adulterants. Thirty-nine known OHAs, including 10 methoxyflavones, 13 coumarins, and 16 furanocoumarins, as well as 13 tentatively screened OHAs, were analyzed in orange, mandarin, grapefruit and pomelo juices using our newly developed high-resolution HPLC-UV and fluorescence detection method. Quantitative OHA profiles from 158 pure juice samples were obtained to establish a purity discriminant model using an omics strategy. Reduction of OHA variables showed that three important methoxyflavones, i.e. isosinensetin, tangeretin and sinensetin provided the best discrimination ability between sweet orange and mandarin juices. There are two subtypes of pomelos, Shatianyou Group and Wendan Group, of which juices should be separately compared to grapefruit juice. Five OHAs, namely meranzin, 3,5,6,7,8,3\',4\'-heptamethoxyflavone, osthole, 6\',7\'-epoxybergamottin, and bergamottin were found to discriminate Shatianyou Group of pomelo juice from grapefruit juice; whereas three OHAs, namely bergaptol, isomeranzin, and 6\',7\'-dihydroxybergamottin were able to discriminate Wendan Group of pomelo juice from grapefruit juice. The established partial least squares discriminant analysis (PLS-DA) models were capable of detecting as little as 10% mandarin juice in sweet orange juice and 10% pomelo juice in grapefruit juice, allowing for fast prescreening of excess addition with good reliability (root mean square error of prediction, RMSEP < 5%).