背景:microRNAs(miRNAs)很小,非编码RNA分子,作为癌基因或肿瘤抑制基因。miRNA中的SNP可能会改变与miRNA相关的基因的表达,增强对乳腺癌的易感性。miRNA-146a(rs2910164)和miRNA-196a(rs11614913)均被鉴定,并且与多个种族的乳腺癌风险显着相关。但在巴基斯坦的开伯尔·普赫图赫瓦人口中仍未被开发。
方法:本研究旨在检查所选SNP与乳腺癌风险的关系。研究队列包括100名乳腺癌患者和100名健康对照。对所有参与者进行DNA提取,然后进行T-ARMSPCR和凝胶电泳。
结果:结果显示miRNA-146a的风险等位基因(G)与乳腺癌风险增加之间存在强关联(OR=2.04,P=0.0006)。同样,杂合基因型和突变基因型也显示了高风险和与乳腺癌风险显著相关(CG;OR=0.51,9P=0.0001)(GG;OR=3.76,P=0.04).然而,miRNA-196a(rs11614913)的风险等位基因(T)未能显示与乳腺癌风险显著相关(OR=0.92P=0.68).同样,杂合子和突变基因型与乳腺癌风险无显著相关性(CT;OR=0.52,P=0.125(TT;OR=0.88,P=0.84).此外,miRNA-146a(rs2910164)和miRNA-196a(rs11614913)多态性与家族史无显著关联(P=0.34,P=0.77),PR状态(P=0.310,P=0.397),ER状态(P=0.992,P=0.981),节点状态(P=0.86,P=0.90),月经状态(P=0.97,P=0.09)。值得注意的是,miRNA-196a与转移组(P=0.010)和癌症分期(P=0.047)显著相关。
结论:结论:这项研究强调了miRNA-146a(rs2910164)多态性与乳腺癌风险的关联,但提示miRNA-196a(rs11614913)与乳腺癌风险无显著关联.然而,这些发现需要通过更大的数据集来证实,以获得更准确的结果.
BACKGROUND: microRNAs (miRNAs) are small, noncoding RNA molecules, functioning either as oncogenes or tumor suppressor genes. SNPs in miRNAs might modify the expression of genes associated with miRNAs, enhancing susceptibility to breast cancer. Both miRNA-146a (rs2910164) and miRNA-196a (rs11614913) are identified and significantly associated with breast cancer risk in several ethnicities, but remains unexplored in Khyber Pakhtunkhwa population of Pakistan.
METHODS: This study was aimed to check the relation of selected SNPs with breast cancer risk. The research cohort included 100 breast cancer patients and 100 healthy controls. All the participants were subjected for DNA extraction followed by T-ARMS PCR and gel electrophoresis.
RESULTS: The results revealed a strong association between risk allele (G) of miRNA-146a and increased risk of breast cancer (OR = 2.04, P = 0.0006). Similarly, heterozygous and mutant genotypes also indicated high risk and significant association with breast cancer risk (CG; OR = 0.51, 9 P = 0.0001) (GG; OR = 3.76, P = 0.04). However, risk allele (T) of miRNA-196a (rs11614913) failed to exhibit significant association with breast cancer risk (OR = 0.92 P = 0.68). Similarly, the heterozygous and mutant genotype did not show significant association with breast cancer risk (CT; OR = 0.52, P = 0.125 (TT; OR = 0.88, P = 0.84). Furthermore, miRNA-146a (rs2910164) and miRNA-196a (rs11614913)
polymorphisms exhibited non-significant associations with family history (P = 0.34, P = 0.77), PR status (P = 0.310, P = 0.397), ER status (P = 0.992, P = 0.981), nodal status (P = 0.86, P = 0.90), and menstrual status (P = 0.97, P = 0.09). Notably, miRNA-196a showed a significant association with the metastasis group (P = 0.010) and cancer stages (P = 0.047).
CONCLUSIONS: In conclusion, this study highlights the association of miRNA-146a (rs2910164) polymorphism with breast cancer risk but suggested non-significant association of miRNA-196a (rs11614913) with breast cancer risk. However, these findings need to be confirmed through larger data set for more accurate result.