Polymerase chain reaction and Sanger sequencing were used to detect the genotype of FOXP3 gene rs3761548 and rs2232365 polymorphisms.
Smoking, diabetes mellitus (DM), and HBP histories, higher TG and HDL-C levels were more frequently observed in IS patients than in controls. In comparison with rs3761548 GG genotype, GT genotype (OR = 1.573, 95 %CI = 1.030-2.402; adjusted: OR = 1.736, 95 %CI = 1.070-2.817) and GT + TT vs. GG model (OR = 1.581, 95 %CI = 1.0449-2.382; adjusted: OR = 1.720, 95 %CI = 1.074-2.755) of rs3761548 polymorphism was significantly correlated with elevated ischemic stroke susceptibility both at prior and after adjusted by smoking, HBP, DM, TG and HDL-C. Recessive model of rs2232365 polymorphism could elevate the susceptibility of ischemic stroke (OR = 11.962, 95 %CI = 1.144-3.3363; adjusted: OR = 1.876, 95 %CI = 1.016-3.463). Besides, rs3761548 dominant model (OR = 2.757, 95 %CI = 1.379-5.552; adjusted: OR = 2.601, 95 %CI = 1.268-5.336) and rs2232365 recessive model (OR = 3.103, 95 %CI = 1.463-6.583; adjusted: OR = 3.545, 95 %CI = 1.600-7.855) were related to the severity of ischemic stroke.
FOXP3 gene rs3761548 and rs2232365 polymorphisms were risk factors for susceptibility and severity of IS.
方法:采用聚合酶链反应和Sanger测序法检测FOXP3基因rs3761548和rs222365多态性的基因型。
结果:吸烟,糖尿病(DM),和HBP历史,IS患者的TG和HDL-C水平高于对照组.与rs3761548GG基因型相比,GT基因型(OR=1.573,95CI=1.030-2.402;调整后:OR=1.736,95CI=1.070-2.817)和GTTTvs.rs3761548多态性的GG模型(OR=1.581,95CI=1.0449-2.382;校正:OR=1.720,95CI=1.074-2.755)与吸烟前后缺血性卒中易感性升高显着相关,HBP,DM,TG和HDL-Crs2232365多态性的隐性模型可以提高缺血性卒中的易感性(OR=11.962,95CI=1.144-3.3363;校正:OR=1.876,95CI=1.016-3.463)。此外,rs3761548显性模型(OR=2.757,95CI=1.379-5.552;校正:OR=2.601,95CI=1.268-5.336)和rs222365隐性模型(OR=3.103,95CI=1.463-6.583;校正:OR=3.545,95CI=1.600-7.855)与缺血性卒中严重程度相关。
结论:FOXP3基因rs3761548和rs2232365多态性是IS易感性和严重程度的危险因素。