Abstract：
Immunoinflammatory response plays an important role in the pathophysiological process of ischemic stroke (IS). Forkhead box P3 (FOXP3) is a master regulator for immune cells. Polymorphisms of FOXP3 gene might contribute to the susceptibility of IS. This study aimed to explore the association between FOXP3 gene polymorphisms (rs3761548 and rs2232365) and IS susceptibility in the Chinese Han population.
Polymerase chain reaction and Sanger sequencing were used to detect the genotype of FOXP3 gene rs3761548 and rs2232365 polymorphisms.
Smoking, diabetes mellitus (DM), and HBP histories, higher TG and HDL-C levels were more frequently observed in IS patients than in controls. In comparison with rs3761548 GG genotype, GT genotype (OR = 1.573, 95 %CI = 1.030-2.402; adjusted: OR = 1.736, 95 %CI = 1.070-2.817) and GT + TT vs. GG model (OR = 1.581, 95 %CI = 1.0449-2.382; adjusted: OR = 1.720, 95 %CI = 1.074-2.755) of rs3761548 polymorphism was significantly correlated with elevated ischemic stroke susceptibility both at prior and after adjusted by smoking, HBP, DM, TG and HDL-C. Recessive model of rs2232365 polymorphism could elevate the susceptibility of ischemic stroke (OR = 11.962, 95 %CI = 1.144-3.3363; adjusted: OR = 1.876, 95 %CI = 1.016-3.463). Besides, rs3761548 dominant model (OR = 2.757, 95 %CI = 1.379-5.552; adjusted: OR = 2.601, 95 %CI = 1.268-5.336) and rs2232365 recessive model (OR = 3.103, 95 %CI = 1.463-6.583; adjusted: OR = 3.545, 95 %CI = 1.600-7.855) were related to the severity of ischemic stroke.
FOXP3 gene rs3761548 and rs2232365 polymorphisms were risk factors for susceptibility and severity of IS.
Polymerase chain reaction and Sanger sequencing were used to detect the genotype of FOXP3 gene rs3761548 and rs2232365 polymorphisms.
Smoking, diabetes mellitus (DM), and HBP histories, higher TG and HDL-C levels were more frequently observed in IS patients than in controls. In comparison with rs3761548 GG genotype, GT genotype (OR = 1.573, 95 %CI = 1.030-2.402; adjusted: OR = 1.736, 95 %CI = 1.070-2.817) and GT + TT vs. GG model (OR = 1.581, 95 %CI = 1.0449-2.382; adjusted: OR = 1.720, 95 %CI = 1.074-2.755) of rs3761548 polymorphism was significantly correlated with elevated ischemic stroke susceptibility both at prior and after adjusted by smoking, HBP, DM, TG and HDL-C. Recessive model of rs2232365 polymorphism could elevate the susceptibility of ischemic stroke (OR = 11.962, 95 %CI = 1.144-3.3363; adjusted: OR = 1.876, 95 %CI = 1.016-3.463). Besides, rs3761548 dominant model (OR = 2.757, 95 %CI = 1.379-5.552; adjusted: OR = 2.601, 95 %CI = 1.268-5.336) and rs2232365 recessive model (OR = 3.103, 95 %CI = 1.463-6.583; adjusted: OR = 3.545, 95 %CI = 1.600-7.855) were related to the severity of ischemic stroke.
FOXP3 gene rs3761548 and rs2232365 polymorphisms were risk factors for susceptibility and severity of IS.
摘要：
目的：免疫炎症反应在缺血性卒中的病理生理过程中起重要作用。叉头盒P3(FOXP3)是免疫细胞的主要调控因子。FOXP3基因多态性可能与IS的易感性有关。本研究旨在探讨FOXP3基因多态性(rs3761548和rs2232365)与中国汉族人群IS易感性的关系。
方法：采用聚合酶链反应和Sanger测序法检测FOXP3基因rs3761548和rs222365多态性的基因型。
结果：吸烟，糖尿病（DM），和HBP历史，IS患者的TG和HDL-C水平高于对照组.与rs3761548GG基因型相比，GT基因型（OR=1.573，95CI=1.030-2.402；调整后：OR=1.736，95CI=1.070-2.817）和GTTTvs.rs3761548多态性的GG模型（OR=1.581，95CI=1.0449-2.382；校正：OR=1.720，95CI=1.074-2.755）与吸烟前后缺血性卒中易感性升高显着相关，HBP,DM,TG和HDL-Crs2232365多态性的隐性模型可以提高缺血性卒中的易感性（OR=11.962，95CI=1.144-3.3363；校正：OR=1.876，95CI=1.016-3.463）。此外,rs3761548显性模型（OR=2.757，95CI=1.379-5.552；校正：OR=2.601，95CI=1.268-5.336）和rs222365隐性模型（OR=3.103，95CI=1.463-6.583；校正：OR=3.545，95CI=1.600-7.855）与缺血性卒中严重程度相关。
结论：FOXP3基因rs3761548和rs2232365多态性是IS易感性和严重程度的危险因素。
方法：采用聚合酶链反应和Sanger测序法检测FOXP3基因rs3761548和rs222365多态性的基因型。
结果：吸烟，糖尿病（DM），和HBP历史，IS患者的TG和HDL-C水平高于对照组.与rs3761548GG基因型相比，GT基因型（OR=1.573，95CI=1.030-2.402；调整后：OR=1.736，95CI=1.070-2.817）和GTTTvs.rs3761548多态性的GG模型（OR=1.581，95CI=1.0449-2.382；校正：OR=1.720，95CI=1.074-2.755）与吸烟前后缺血性卒中易感性升高显着相关，HBP,DM,TG和HDL-Crs2232365多态性的隐性模型可以提高缺血性卒中的易感性（OR=11.962，95CI=1.144-3.3363；校正：OR=1.876，95CI=1.016-3.463）。此外,rs3761548显性模型（OR=2.757，95CI=1.379-5.552；校正：OR=2.601，95CI=1.268-5.336）和rs222365隐性模型（OR=3.103，95CI=1.463-6.583；校正：OR=3.545，95CI=1.600-7.855）与缺血性卒中严重程度相关。
结论：FOXP3基因rs3761548和rs2232365多态性是IS易感性和严重程度的危险因素。
