UNASSIGNED:肺动脉高压(PH)大鼠模型的成功主要取决于肺动脉压的测量。我们在此展示了一种通过高频超声引导的经胸穿刺测量大鼠肺动脉压的新方法。还讨论了这种新方法的功效和时间。
UNASSIGNED:单次皮下注射野百合碱(MCT)以建立大鼠PH模型。通过热成型方法,在取出针芯后,穿刺套管的尖端保持一定角度.在胸骨旁主动脉的短轴部分进行了右心室流出道的平面内实时引导套管针穿刺。利用外部压力传感器记录右心室收缩压的实时波形,肺动脉收缩压,和舒张压.
UNASSIGNED:在模型组和对照组中使用这种新方法的成功率分别为88.5%和86.7%,分别。穿刺压力测量时间为164±31和235±50s,分别。右心室收缩压,肺收缩压,模型组舒张压高于对照组。
UNASSIGNED:套管针的改良方法有助于精确定位肺动脉测压。该方法成功率高,手术时间短。它可以同时测量收缩压,舒张压,右心室和肺动脉的平均压力。在验证年夜鼠PH模子和肺动脉压监测方面具有辽阔的运用远景。
UNASSIGNED: The success of the rat model of pulmonary hypertension (PH) is primarily dependent on the measurement of pulmonary artery pressure. We herein demonstrate a novel method for measuring pulmonary artery pressure through a high-frequency ultrasound-guided transthoracic puncture in rats. The efficacy and time of this novel method are also discussed.
UNASSIGNED: A single subcutaneous injection of monocrotaline (MCT) was used to establish a rat model of PH. Through the heat shaping method, the tip of that puncture cannula was maintained at a certain angle after the needle core was removed. In-plane real-time guided trocar puncture of the right ventricular outflow tract was performed in the short-axis section of the parasternal aorta. The external pressure sensor was used to record the real-time waveform of right ventricular systolic pressure, pulmonary artery systolic pressure, and diastolic pressure.
UNASSIGNED: The success rates of which using this novel method in the model group and the control group were 88.5 and 86.7%, respectively. The time of puncture pressure measurement was 164 ± 31 and 235 ± 50 s, respectively. The right ventricular systolic blood pressure, pulmonary systolic blood pressure, and diastolic blood pressure of the model group were higher than those of the control group.
UNASSIGNED: The modified method for trocar is helpful for accurately positioning pulmonary artery manometry. The method described in this paper has a high success rate and short operation time. It can simultaneously measure systolic blood pressure, diastolic blood pressure, and mean pressure of the right ventricle and pulmonary artery. It has a broad application prospect in verifying the rat PH model and pulmonary artery pressure monitoring.